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- Table of Contents
IRAK1 antibody (A01021-2) performs well in ELISA, IP, IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescence Validation of IRAK in Human HeLa Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa Cells labeling IRAK with A01021-2 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa Cells labeling IRAK with A01021-2 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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Anti-IRAK IRAK1 Antibody
IRAK1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IP, IF, ICC, WB |
Recommend Dilution
Application
Dilution
Species
WB: 1-4 μg/mL; IF: 20 μg/mL; ICC: 10 μg/mL.
Antibody validated: Western Blot in human
mouse and rat samples; Immunofluorescence and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-IRAK IRAK1 Antibody
View all IRAK1 Antibodies
View all IRAK1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
IRAK antibody can be stored at 4°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
IRAK Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-IRAK antibody was raised against a peptide corresponding to 13 amino acids near the carboxy terminus of human IRAK. The immunogen is located within the last 50 amino acids of IRAK.
Cross-Reactivity
At least four isoforms of IRAK are known to exist; this antibody will detect all four isoforms. IRAK antibody is predicted to not cross-react with other members of the IRAK protein family.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
76.5 kDa
Background of IRAK1
Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently. IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response. IRAK mediates a signaling cascade leading to NF-κB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF).
Cite This Product
Anti-IRAK IRAK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01021-2)
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Product Promise
We promise Anti-IRAK IRAK1 Antibody performs excellently in relevant applications for ELISA, IP, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01021-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026