Anti-IRBP/RBP3 Antibody Picoband™

Product Name

Anti-IRBP/RBP3 Antibody Picoband™

View all RBP3 Antibodies

SKU/Catalog Number

A02913-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-IRBP/RBP3 Antibody Picoband™ catalog # A02913-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-IRBP/RBP3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02913-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human IRBP/RBP3 recombinant protein (Position: L333-R1196).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02913-1 is reactive to RBP3 in Human, Mouse, Rat

Applications

A02913-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

135 kDa

Calculated molecular weight

135.363kDa

Background of RBP3

Retinol-binding protein 3, interstitial (RBP3), also known as IRBP is a protein that in humans is encoded by the RBP3 gene. Interphotoreceptor retinol-binding protein is a large glycoprotein known to bind retinoids and found primarily in the interphotoreceptor matrix of the retina between the retinal pigment epithelium and the photoreceptor cells. It is thought to transport retinoids between the retinal pigment epithelium and the photoreceptors, a critical role in the visual process.The human IRBP gene is approximately 9.5 kbp in length and consists of four exons separated by three introns. The introns are 1.6-1.9 kbp long. The gene is transcribed by photoreceptor and retinoblastoma cells into an approximately 4.3-kilobase mRNA that is translated and processed into a glycosylated protein of 135,000 Da. The amino acid sequence of human IRBP can be divided into four contiguous homology domains with 33-38% identity, suggesting a series of gene duplication events. In the gene, the boundaries of these domains are not defined by exon-intron junctions, as might have been expected. The first three homology domains and part of the fourth are all encoded by the first large exon, which is 3,180 base pairs long. The remainder of the fourth domain is encoded in the last three exons, which are 191, 143, and approximately 740 base pairs long, respectively.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of IRBP/RBP3 using anti-IRBP/RBP3 antibody (A02913-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat eye tissue lysates,
Lane 2: mouse eye tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRBP/RBP3 antigen affinity purified polyclonal antibody (Catalog # A02913-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRBP/RBP3 at approximately 135 kDa. The expected band size for IRBP/RBP3 is at 135 kDa.

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Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-IRBP/RBP3 antibody (A02913-1).
Overlay histogram showing MCF-7 cells stained with A02913-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IRBP/RBP3 Antibody (A02913-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For RBP3 (Source: Uniprot.org, NCBI)

Alternative Names

D10S64; D10S65; D10S66; Interphotoreceptor retinoid-binding protein; Interstitial retinol-binding protein; IRBP; RBPI; retinol binding protein 3, interstitial; retinol-binding protein 3; retinol-binding protein 3, interstitial RBP3 D10S64, D10S65, D10S66, IRBP, RBPI, RP66 retinol binding protein 3 retinol-binding protein 3|interphotoreceptor retinoid-binding protein|interstitial retinol binding protein 3|retinol binding protein 3, interstitial

For more info on RBP3, check out the RBP3 Infographic

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In this infographic, you will see the following information for RBP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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