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Product Info Summary
| SKU: | RP1077 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-JNK2/MAPK9 Antibody
SKU/Catalog Number
RP1077
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-JNK2/MAPK9 Antibody catalog # RP1077. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-JNK2/MAPK9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1077)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human JNK2, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1077 is reactive to MAPK9 in Human
Applications
RP1077 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
48 kDa
Calculated molecular weight
48.139kDa
Background of JNK2
JNK2 is also known as MAPK9. The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. Also, this gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-JNK2 antibody, RP1077, Western blotting
All lanes: Anti JNK2 (RP1077) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 48KD
Observed bind size: 48KD
Protein Target Info & Infographic
Gene/Protein Information For MAPK9 (Source: Uniprot.org, NCBI)
Gene Name
MAPK9
Full Name
Mitogen-activated protein kinase 9
Weight
48.139kDa
Superfamily
protein kinase superfamily
Alternative Names
c-Jun kinase 2; c-Jun N-terminal kinase 2; EC 2.7.11; EC 2.7.11.24; JNK2; JNK2A; JNK2BETA; JNK2JNK2ALPHA; JNK-55; Jun kinase; MAP kinase 9; MAPK 9; MAPK9; mitogen-activated protein kinase 9; p54a; p54aSAPK; PRKM9JNK2B; SAPK; SAPK1 alpha; Stress-activated protein kinase JNK2 MAPK9 JNK-55, JNK2, JNK2A, JNK2ALPHA, JNK2B, JNK2BETA, PRKM9, SAPK, SAPK1a, p54a, p54aSAPK mitogen-activated protein kinase 9 mitogen-activated protein kinase 9|Jun kinase|MAP kinase 9|MAPK 9|c-Jun N-terminal kinase 2|c-Jun kinase 2|stress-activated protein kinase 1a|stress-activated protein kinase JNK2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAPK9, check out the MAPK9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAPK9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-JNK2/MAPK9 Antibody (RP1077)
Hello CJ!
RP1077 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Recombinant Newcastle disease virus (rL-RVG) triggers autophagy and apoptosis in gastric carcinoma cells by inducing ER stress
Wang P, Mao Z, Pan Q, Lu R, Huang X, Shang X, Zhang R, You H. Int J Mol Med. 2018 Apr;41(4):2117-2127. doi: 10.3892/ijmm.2018.3410. Epub 2018 Jan 22. Histone deacetylase-4 and histone deacetylase-8 regulate interleukin-1β-induced cartilage catabol...
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-JNK2/MAPK9 Antibody
Question
We are currently using anti-JNK2/MAPK9 antibody RP1077 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
N. Parker
Verified customer
Asked: 2019-08-28
Answer
The anti-JNK2/MAPK9 antibody (RP1077) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-28
