Anti-Keratin KRT6C Monoclonal Antibody

Product Name

Anti-Keratin KRT6C Monoclonal Antibody

View all KRT6C Antibodies

SKU/Catalog Number

M07078

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Keratin KRT6C Monoclonal Antibody (Catalog # M07078). Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Keratin KRT6C Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07078)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: C11

Isotype

IgG1

Immunogen

This protein A purified monoclonal antibody was produced by repeated immunizations with purified human cytoskeletal preparations from A431 cells.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M07078 is reactive to KRT6C in Human

Applications

M07078 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

60.025kDa

Background of KRT6C

Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. There are two types of keratins (cytoskeletal and microfibrillar) and are clustered in a region of chromosome. Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (i.e. skin) and non-keratinizing cells (i.e. mesothelial). Anti-Keratin Antibody is useful for researchers interested in cytoskeletal signaling and developmental biology research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000 - 1:20,000
IHC: 1:50 - 1:200
IF Microscopy: 1:50 - 1:200
IP: 1:100
WB: 1:50 - 1:200

Validation Images & Assay Conditions

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Immunofluorescence Microscopy of Immunochemical's Anti-Keratin antibody was used with Dylight 488 goat anti-mouse IgG (shown in green) to detect Keratin by Immunofluorescence. In the same experiment, Anti-HDAC-1 polyclonal antibody was used with Anti-Rabbit IgG (shown in red) to detect HDAC-1. Myriam Gastard, PhD, personal communication, Leica Microsystems, Inc. USA.

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Western blot analysis of Keratin expression in human epidermis (lane 1). Keratin at 56KD was detected using mouse anti-Keratin protein A purified monoclonal antibody (Catalog # M07078) at 1:400. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1001).

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Keratin was detected in immunofluorescence sections of HeLa Whole Cell using mouse anti-Keratin protein A purified monoclonal antibody (Catalog # M07078). Confocal slices of HeLa cells are between 0.5 and 0.6

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Keratin was detected in paraffin-embedded sections of human prostate tissues using mouse anti-Keratin protein A purified monoclonal antibody (Catalog # M07078) at 10 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1021).

Gene/Protein Information For KRT6C (Source: Uniprot.org, NCBI)

Alternative Names

Keratin, type II cytoskeletal 6C KRT6C K6E, KRT6E, PPKNEFD keratin 6C keratin, type II cytoskeletal 6C|CK-6C|CK-6E|K6C|cytokeratin-6C|cytokeratin-6E|keratin 6C, type II|keratin 6E|keratin K6h|type-II keratin Kb12

For more info on KRT6C, check out the KRT6C Infographic

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In this infographic, you will see the following information for KRT6C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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