Anti-LAMA3/Laminin 5 Rabbit Monoclonal Antibody

LAMA3 antibody

Boster Bio Anti-LAMA3/Laminin 5 Rabbit Monoclonal Antibody catalog # M04194. Tested in WB, ICC/IF applications. This antibody reacts with Human.

Product Info Summary

SKU: M04194
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: IF, ICC, WB

Product Name

Anti-LAMA3/Laminin 5 Rabbit Monoclonal Antibody

View all LAMA3 Antibodies

SKU/Catalog Number

M04194

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-LAMA3/Laminin 5 Rabbit Monoclonal Antibody catalog # M04194. Tested in WB, ICC/IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-LAMA3/Laminin 5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04194)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AOAB-12

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human LAMA3

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M04194 is reactive to LAMA3 in Human

Applications

M04194 is guaranteed for IF, ICC, WB Boster Guarantee

Observed Molecular Weight

70 kDa

Calculated molecular weight

366.649kDa

Background of LAMA3

Plays a major role in tight junction-specific obliteration of the intercellular space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For LAMA3 (Source: Uniprot.org, NCBI)

Gene Name

LAMA3

Full Name

Laminin subunit alpha-3

Weight

366.649kDa

Alternative Names

Epiligrin; LAMA3; Laminin alpha 3; Laminin5; Laminin-5 LAMA3 BM600, E170, LAMNA, LOCS laminin subunit alpha 3 laminin subunit alpha-3|BM600 150kD subunit|LAM3, alpha-3 subunit|epiligrin 170 kda subunit|epiligrin alpha 3 subunit|kalinin 165kD subunit|kalinin subunit alpha|laminin 5, alpha-3 subunit|laminin A3|laminin, alpha 3 (nicein (150kD), kalinin (165kD), BM600 (150kD), epilegrin)|laminin-5 alpha 3 chain|laminin-5 subunit alpha|laminin-6 subunit alpha|laminin-7 subunit alpha|nicein 150kD subunit|nicein subunit alpha

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on LAMA3, check out the LAMA3 Infographic

LAMA3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LAMA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-LAMA3/Laminin 5 Rabbit Monoclonal Antibody

Question

We are currently using anti-LAMA3/Laminin 5 Rabbit Monoclonal antibody M04194 for human tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-24

Answer

The anti-LAMA3/Laminin 5 Rabbit Monoclonal antibody (M04194) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-24

Question

Is this M04194 anti-LAMA3/Laminin 5 Rabbit Monoclonal antibody reactive to the isotypes of LAMA3?

Verified Customer

Verified customer

Asked: 2019-09-25

Answer

The immunogen of M04194 anti-LAMA3/Laminin 5 Rabbit Monoclonal antibody is A synthesized peptide derived from human LAMA3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-09-25

Question

I was wanting to use your anti-LAMA3/Laminin 5 Rabbit Monoclonal antibody for IF for human ectocervix on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human ectocervix identification?

Verified Customer

Verified customer

Asked: 2019-08-07

Answer

It shows on the product datasheet, M04194 anti-LAMA3/Laminin 5 Rabbit Monoclonal antibody has been tested for IF, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human ectocervix in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-07

Question

I am interested in to test anti-LAMA3/Laminin 5 Rabbit Monoclonal antibody M04194 on human ectocervix for research purposes, then I may be interested in using anti-LAMA3/Laminin 5 Rabbit Monoclonal antibody M04194 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-04-22

Answer

The products we sell, including anti-LAMA3/Laminin 5 Rabbit Monoclonal antibody M04194, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-04-22

Question

Is a blocking peptide available for product anti-LAMA3/Laminin 5 Rabbit Monoclonal antibody (M04194)?

Verified Customer

Verified customer

Asked: 2018-11-12

Answer

We do provide the blocking peptide for product anti-LAMA3/Laminin 5 Rabbit Monoclonal antibody (M04194). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-11-12

Question

I have a question about product M04194, anti-LAMA3/Laminin 5 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

H. Bhatt

Verified customer

Asked: 2017-05-09

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M04194 anti-LAMA3/Laminin 5 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-05-09

Order DetailsPrice
M04194

100μL

$370
M04194-30ul

30μL sample

$99
M04194-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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M04194
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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