Anti-LAMP2 Monoclonal Antibody

LAMP-2/CD107b antibody

Boster Bio Anti-LAMP2 Monoclonal Antibody catalog # M01573-1. Tested in IP, IF, WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: M01573-1
Size: 100ug
Reactive Species: Human, Mouse, Rabbit
Host: Rat
Application: IP, IF, ICC, WB

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Product Name

Anti-LAMP2 Monoclonal Antibody

View all LAMP-2/CD107b Antibodies

SKU/Catalog Number

M01573-1

Size

100ug

Form

liquid

Description

Boster Bio Anti-LAMP2 Monoclonal Antibody catalog # M01573-1. Tested in IP, IF, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-LAMP2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01573-1)

Host

Rat

Contents

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Clonality

Monoclonal

Clone Number

GL2A7

Isotype

IgG1

Immunogen

Purified preparation of mouse liver lysosomal membranes

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~100-110kDa.

Reactive Species

M01573-1 is reactive to LAMP2 in Human, Mouse, Rabbit

Applications

M01573-1 is guaranteed for IP, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

44.961kDa

Background of LAMP-2/CD107b

Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37% sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). LAMP2 has also been detected at the plasma membrane of cells, as well as in cells that secrete lysosomal hydrolases. A study in the developmental expresses patterns of membrane LAMP2 transcripts indicate a possible involvement of this protein in cell-cell or cell-extracellular matrix interaction, and appear to reflect tissue and cell type specific roles of lysosomes during morphogenesis (4). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxyl-terminal tyrosine based motif (YXXI) (5). This stimulation has also been shown to have an associated release of histamine, leukotriene C4 and prostaglandin D2, which shows that LAMP1 and LAMP2 are activation markers for normal mast cells (5). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (6). LAMP2 has also been shown to be critical for autophagy, in conversion of early autophagic vacuoles to vacuoles which rapidly degrade their content (7).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB (1:1000), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.

Validation Images & Assay Conditions

Gene/Protein Information For LAMP2 (Source: Uniprot.org, NCBI)

Gene Name

LAMP2

Full Name

Lysosome-associated membrane glycoprotein 2

Weight

44.961kDa

Superfamily

LAMP family

Alternative Names

CD107 antigen-like family member B; CD107b antigen; CD107b; LAMP2; LAMP-2; LAMPB; LGP110; LGP-96; Lysosomal Associated Membrane Protein 2 ; lysosomal-associated membrane protein 2; lysosome-associated membrane glycoprotein 2; Lysosome-associated membrane protein 2 LAMP2 CD107b, DND, LAMP-2, LAMPB, LGP-96, LGP110 lysosomal associated membrane protein 2 lysosome-associated membrane glycoprotein 2|CD107 antigen-like family member B

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on LAMP2, check out the LAMP2 Infographic

LAMP2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LAMP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-LAMP2 Monoclonal Antibody

Question

Will M01573-1 anti-LAMP2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-02-11

Answer

You can see on the product datasheet, M01573-1 anti-LAMP2 Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-02-11

Question

Will anti-LAMP2 Monoclonal antibody M01573-1 work on pig IP with plasma?

Verified Customer

Verified customer

Asked: 2019-12-24

Answer

Our lab technicians have not tested anti-LAMP2 Monoclonal antibody M01573-1 on pig. You can run a BLAST between pig and the immunogen sequence of anti-LAMP2 Monoclonal antibody M01573-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig plasma in IP, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-24

Question

See below the WB image, lot number and protocol we used for lymph using anti-LAMP2 Monoclonal antibody M01573-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-16

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-16

Question

Do you have a BSA free version of anti-LAMP2 Monoclonal antibody M01573-1 available?

Verified Customer

Verified customer

Asked: 2019-05-23

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-LAMP2 Monoclonal antibody M01573-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-05-23

Question

Does anti-LAMP2 Monoclonal antibody M01573-1 work for IP with lymph?

Verified Customer

Verified customer

Asked: 2018-09-04

Answer

According to the expression profile of lymph, LAMP2 is highly expressed in lymph. So, it is likely that anti-LAMP2 Monoclonal antibody M01573-1 will work for IP with lymph.

Boster Scientific Support

Answered: 2018-09-04

Question

I was wanting to use your anti-LAMP2 Monoclonal antibody for IP for human lymph on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lymph identification?

Verified Customer

Verified customer

Asked: 2018-05-14

Answer

It shows on the product datasheet, M01573-1 anti-LAMP2 Monoclonal antibody has been validated for IP, IF, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human lymph in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-05-14

Question

We are currently using anti-LAMP2 Monoclonal antibody M01573-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on bovine tissues as well?

P. Lewis

Verified customer

Asked: 2013-01-17

Answer

The anti-LAMP2 Monoclonal antibody (M01573-1) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-01-17

$387
Backordered.

Lead time for this item is typically 10-14 days

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M01573-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$387.00

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