Anti-LST1 Purified Monoclonal Antibody

Product Name

Anti-LST1 Purified Monoclonal Antibody

View all LST1 Antibodies

SKU/Catalog Number

M03702

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-LST1 Purified Monoclonal Antibody (Catalog# M03702). Tested in IP, WB, ICC application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-LST1 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03702)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

LST1/02

Isotype

Mouse IgG1

Immunogen

KLH-fused peptide corresponding to amino acids SSEGPDLRGRDKRGT of human LST1. The antibody LST1/02 reacts with an extracellular epitope of LST1, an approximately 6-11 kDa protein expressed as various transmembrane or soluble forms. LST1 is found predominantly on monocytes and dendritic cells. It migrates on SDS PAGE gels as approximately 25-28 kDa molecule.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M03702 is reactive to LST1 in Human

Observed Molecular Weight

42 kDa

Calculated molecular weight

10.8 kDa

Background of LST1

LST1 (leukocyte-specific transcript 1, also known as B144) is expressed in cells of myeloid/erythroid lineage (monocytes, granulocytes, dendritic cells, platelets, erythrocytes). At least 14 alternatively spliced variants (LST1/A –; LST1/N) can be detected; some of them (LST1/A, B, C, G, I, K) are transmembrane cell surface-exposed forms, the other are soluble. LST1 induces production of long, thin filopodia in dendritic cells, has an inhibitory effect on lymphocyte proliferation and may have an immunomodulatory role. LST1/A is an 11 kDa transmembrane adaptor present in membrane rafts and forms spontaneously covalent homodimers. Its intracellular domain contains two tyrosine motifs, one of them being an ITIM very similar to such motifs in Siglec.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M03702 is guaranteed for IP, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunoprecipitation: Positive control: RAJI human Burkitt lymphoma cell line.
Western blotting: The antigen migrates on SDS PAGE gels as approximately 25-28 kDa molecule.

Validation Images & Assay Conditions

See full target information LST1

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