Anti-MARCHF2 Antibody Picoband™

Product Name

Anti-MARCHF2 Antibody Picoband™

View all MARCHF2 Antibodies

SKU/Catalog Number

A13497-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-MARCHF2 Antibody Picoband™ catalog # A13497-2. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-MARCHF2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13497-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human MARCHF2 recombinant protein (Position: M1-V246).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A13497-2 is reactive to MARCHF2 in Human

Applications

A13497-2 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

27 kDa

Calculated molecular weight

26.995kDa

Background of MARCHF2

MARCH2 is an E3 ubiquitin-protein ligase that may mediate ubiquitination of TFRC and CD86, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. MARCH2 may be involved in endosomal trafficking through interaction with STX6.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of MARCHF2 using anti-MARCHF2 antibody (A13497-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MARCHF2 antigen affinity purified polyclonal antibody (Catalog # A13497-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MARCHF2 at approximately 27 kDa. The expected band size for MARCHF2 is at 27 kDa.

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Figure 2. IF analysis of MARCHF2 using anti-MARCHF2 antibody (A13497-2).
MARCHF2 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MARCHF2 Antibody (A13497-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For MARCHF2 (Source: Uniprot.org, NCBI)

Alternative Names

E3 ubiquitin-protein ligase MARCHF2 MARCHF2 HSPC240, MARCH-II, MARCH2, RNF172 membrane associated ring-CH-type finger 2 E3 ubiquitin-protein ligase MARCHF2|E3 ubiquitin-protein ligase MARCH2|RING finger protein 172|RING-type E3 ubiquitin transferase MARCH2|RING-type E3 ubiquitin transferase MARCHF2|membrane associated ring finger 2|membrane-associated RING finger protein 2|membrane-associated RING-CH protein II|membrane-associated ring finger (C3HC4) 2, E3 ubiquitin protein ligase

For more info on MARCHF2, check out the MARCHF2 Infographic

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In this infographic, you will see the following information for MARCHF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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