Anti-MC3 Receptor/MC3R Antibody Picoband™

Product Name

Anti-MC3 Receptor/MC3R Antibody Picoband™

View all Melanocortin-3 R/MC3R Antibodies

SKU/Catalog Number

A02841-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MC3 Receptor/MC3R Antibody Picoband™ catalog # A02841-2. Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MC3 Receptor/MC3R Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02841-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human MC3 Receptor, different from the related mouse sequence by six amino acids, and from the related rat sequence by seven amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02841-2 is reactive to MC3R in Human, Mouse

Applications

A02841-2 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

40 kDa

Calculated molecular weight

36.043kDa

Background of Melanocortin-3 R/MC3R

Melanocortin receptor 3 is a protein that in humans is encoded by the MC3R gene. It is mapped to 20q13.2. This gene encodes a G-protein-coupled receptor for melanocyte-stimulating hormone and adrenocorticotropic hormone that is expressed in tissues other than the adrenal cortex and melanocytes. This gene maps to the same region as the locus for benign neonatal epilepsy. Mice deficient for this gene have increased fat mass despite decreased food intake, suggesting a role for this gene product in the regulation of energy homeostasis. Mutations in this gene are associated with a susceptibility to obesity in humans.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of MC3 Receptor using anti-MC3 Receptor antibody (A02841-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: mouse kidney tissue lysate,
lane 2: COLO320 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MC3 Receptor antigen affinity purified polyclonal antibody (Catalog # A02841-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MC3 Receptor at approximately 40KD. The expected band size for MC3 Receptor is at 36KD.

Gene/Protein Information For MC3R (Source: Uniprot.org, NCBI)

Alternative Names

BMIQ9; MC3; MC3R; MC3-R; melanocortin 3 receptor; melanocortin receptor 3; Melanocortin-3 R; Melanocortin3R; Melanocortin-3R; OB20; Obesity quantitative trait locus; OQTL MC3R BMIQ9, MC3, MC3-R, OB20, OQTL melanocortin 3 receptor melanocortin receptor 3|obesity quantitative trait locus

For more info on MC3R, check out the MC3R Infographic

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In this infographic, you will see the following information for MC3R: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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