Anti-Melanoma-associated antigen 4 MAGEA4 Antibody

Product Name

Anti-Melanoma-associated antigen 4 MAGEA4 Antibody

View all MAGEA4 Antibodies

SKU/Catalog Number

A07175

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Melanoma-associated antigen 4 MAGEA4 Antibody (Catalog # A07175). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

MAGEA4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-Melanoma-associated antigen 4 MAGEA4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07175)

Host

Rabbit

Contents

MAGEA4 antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

MAGEA4 antibody was raised against a 16 amino acid peptide near the amino terminus of human MAGEA4. The immunogen is located within the first 50 amino acids of MAGEA4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

MAGEA4 antibody is human specific. At least four isoforms of MAGEA4 are known to exist.

Reactive Species

A07175 is reactive to MAGEA4 in Human, Mouse, Rat

Applications

A07175 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

34.899kDa

Background of MAGEA4

MAGEA4 is a member of the melanoma-associated antigen family that consists of a number of antigens recognized by cytotoxic T lymphocytes (1). MAGEA4 may serve as a target for antitumoral vaccination and may play a role in embryonal development and tumor transformation or aspects of tumor progression. It is expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma and have been implicated in some hereditary disorders, such as dyskeratosis congenital (2-4).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

MAGEA4 antibody can be used for detection of MAGEA4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of MAGEA4 in A431 cell lysate with MAGEA4 antibody at 1 μg/ml.

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Immunohistochemistry of MAGEA4 in human breast tumor with MAGEA4 antibody at 5 μg/mL.

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Immunofluorescence of MAGEA4 in human breast cancer tissue with MAGEA4 antibody at 20 μg/ml.

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Immunofluorescence of MAGEA4 in human breast cancer tissue with MAGEA4 antibody at 20 μg/ml.

Green: MAGEA4 Antibody (A07175)
Blue: DAPI staining

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Immunocytochemistry of MAGEA4 in HeLa cells with MAGEA4 antibody at 5 μg/ml.

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Immunofluorescence of MAGEA4 inHeLa cells with MAGEA4 antibody at 20 μg/ml.

Red: MAGEA4 Antibody (A07175)
Blue: DAPI staining

Gene/Protein Information For MAGEA4 (Source: Uniprot.org, NCBI)

Alternative Names

CT1.4; MAGE-41; MAGE4A; MAGE4B; MAGEA4; MAGE-X2 antigen; MAGE-X2; melanoma antigen family A, 4; melanoma-associated antigen 4; member 4 MAGEA4 CT1.4, MAGE-41, MAGE-X2, MAGE4, MAGE4A, MAGE4B MAGE family member A4 melanoma-associated antigen 4|MAGE-4 antigen|MAGE-41 antigen|MAGE-X2 antigen|cancer/testis antigen 1.4|cancer/testis antigen family 1, member 4|melanoma antigen family A, 4|melanoma antigen family A4

For more info on MAGEA4, check out the MAGEA4 Infographic

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In this infographic, you will see the following information for MAGEA4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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