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Product Info Summary
| SKU: | A01387 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-MIB1 Antibody
SKU/Catalog Number
A01387
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-MIB1 Antibody (Catalog # A01387). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
MIB1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-MIB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01387)
Host
Rabbit
Contents
MIB1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
MIB1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human MIB1. The immunogen is located within amino acids 920 - 970 of MIB1.
Cross-reactivity
MIB1 antibody is human, mouse and rat reactive. At least three isoforms of MIB1 are known to exist.
Reactive Species
A01387 is reactive to MIB1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
110.1 kDa
Background of MIB1
The E3 ubiquitin-protein ligase MIB1, also known as Mindbomb homolog 1, contains multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase (1). MIB1 interacts with and regulates the cellular expression of the death-associated protein kinase 1 (DAPK1) protein by promoting its ubiquitination and degradation (1). It also positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis (2).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01387 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
MIB1 antibody can be used for detection of MIB1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of MIB1 in A431 cell lysate with MIB1 antibody at 1 μg/ml.
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Immunocytochemistry of MIB1 in A431 cells with MIB1 antibody at 5 μg/mL.
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Immunofluorescence of MIB1 in A431 cells with MIB1 antibody at 20 μg/mL.
Specific Publications For Anti-MIB1 Antibody (A01387)
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