Anti-MKK6 (Ab-207) MAP2K6 Antibody

MKK6/MEK6 antibody

Boster Bio Anti-MKK6 (Ab-207) MAP2K6 Antibody (Catalog # A02011-2). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A02011-2
Size: 100 µl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, WB

Product Name

Anti-MKK6 (Ab-207) MAP2K6 Antibody

View all MKK6/MEK6 Antibodies

SKU/Catalog Number

A02011-2

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-MKK6 (Ab-207) MAP2K6 Antibody (Catalog # A02011-2). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MKK6 (Ab-207) MAP2K6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02011-2)

Host

Rabbit

Contents

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Peptide sequence around aa.205~209 (V-D-S-V-A) derived from Human MKK6.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02011-2 is reactive to MAP2K6 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

39 kDa

Calculated molecular weight

32912 MW

Background of MKK6/MEK6

MEK6 is a member of MAPKK protein kinase family. By using degenerate oligonucleotide primers from the conserved kinase domains of MKK3 and MKK4 two human cDNAs and 1 murine cDNA encoding closely related proteins of the MKK family were cloned. The two human clones appear to be different isoforms of the same gene generated by differential splicing: the shorter clone was designated MKK6, encodes a 278-amino acid protein, while the longer clone, designated MKK6b, encodes a 334-amino acid protein. MKK6 is about 80% identical to MKK3 and 40% identical to MKK4. 1.7-kb human MKK6 transcript is highly expressed in skeletal muscle, while an MKK6b-specific probe detected mRNA bands of 1.8, 2.4, and 4.5 kb that are enriched in heart, skeletal muscle, pancreas and liver. MKK6 plays an important role in intracellular signaling pathways leading toward activation of the p38 MAP kinase . MEK6 phosphorylates and activates p38 in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAPK mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis.

Wang W, et al. (2002) Mol Cell Biol ; 22(10): 3389-403.
Raingeaud J, et al. (1996) Mol Cell Biol; 16(3): 1247-55.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02011-2 is guaranteed for IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Predicted MW: 41kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Validation Images & Assay Conditions

Gene/Protein Information For MAP2K6 (Source: Uniprot.org, NCBI)

Gene Name

MAP2K6

Full Name

Dual specificity mitogen-activated protein kinase kinase 6

Weight

32912 MW

Superfamily

protein kinase superfamily

Alternative Names

Pituitary-specific positive transcription factor 1;PIT-1;Growth hormone factor 1;GHF-1;POU1F1;GHF1, PIT1; MAP2K6 MAPKK6, MEK6, MKK6, PRKMK6, SAPKK-3, SAPKK3 mitogen-activated protein kinase kinase 6 dual specificity mitogen-activated protein kinase kinase 6|MAPK/ERK kinase 6|MAPKK 6|MEK 6|SAPK kinase 3|protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6)|stress-activated protein kinase kinase 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MAP2K6, check out the MAP2K6 Infographic

MAP2K6 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MAP2K6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A02011-2

100ul

$360

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A02011-2
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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