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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin).|
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Human MMP-12 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human MMP-12 ELISA Kit PicoKine™ (96 Tests). Quantitate Human MMP12 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human MMP-12 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0950)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: L17-C470
62.5 pg/ml - 4,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0950 is reactive to MMP12 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
EK0950 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0950-CAP||Anti-Human MMP12 Pre-coated 96-well strip microplate||1|
|EK0950-ST||Human MMP12 Standard||2 vials, 10 ng/tube|
|EK0950-DA||Human MMP12 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human MMP-12 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human MMP-12 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of MMP-12 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For MMP12 (Source: Uniprot.Org, NCBI)
peptidase M10A family
EC 3.4.24; hME; HMEEC 188.8.131.52; Macrophage elastase; macrophage metalloelastase; matrix metallopeptidase 12 (macrophage elastase); matrix metalloproteinase 12 (macrophage elastase); Matrix metalloproteinase-12; ME; MGC138506; MME; MMP12; MMP-12 MMP12 HME, ME, MME, MMP-12 matrix metallopeptidase 12 macrophage metalloelastase|matrix metallopeptidase 12 (macrophage elastase)|matrix metalloproteinase 12 (macrophage elastase)*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on MMP12, check out the MMP12 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MMP12: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0950 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Bchir S,Boumiza S,Ben Nasr H,Garrouch A,Kallel I,Tabka Z,Chahed K.Impact of cathepsin D activity and C224T polymorphism (rs17571) on chronic obstructive pulmonary disease: correlations with oxidative and inflammatory markers.Clin Exp Med.2021 Feb 21.doi:1
EK0950 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Li J,Xie Y,Zhao P,Qin Y,Oliver BG,Tian Y,Li S,Wang M,Liu X. A chinese herbal formula ameliorates COPD by inhibiting the inflammatory response via downregulation of p65, JNK, and p38. Phytomedicine. 2021 Jan 20;83:153475.doi:10.1016/j.phymed.2021.153475.Ep
EK0950 usage in article: APP:ELISA, SAMPLE:SERUM AND BALF, DILUTION:NA
Elastin-derived peptides are involved in the processes of human temporomandibular disorder by inducing inflammatory responses in synovial cells
Concomitant elevations of MMP?9, NGAL, proMMP?9/NGAL and neutrophil elastase in serum of smokers with chronic obstructive pulmonary disease
Narimiya T, Wada S, Kanzaki H, Ishikawa M, Tsuge A, Yamaguchi Y, Nakamura Y. J Periodontal Res. 2017 Apr 10. doi: 10.1111/jre.12453. Orthodontic tensile strain induces angiogenesis via type IV collagen degradation by matrix metalloproteinase-12.
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