Human MMP-12 ELISA Kit PicoKine™

Human MMP-12 ELISA Kit PicoKine™ (96 Tests). Quantitate Human MMP12 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml. Cited in 5 publication(s).

Product Info Summary

SKU: EK0950
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin).

Product Name

Human MMP-12 ELISA Kit PicoKine™

See all MMP-12 products

SKU/Catalog Number

EK0950

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human MMP-12 ELISA Kit PicoKine™ (96 Tests). Quantitate Human MMP12 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human MMP-12 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0950)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: L17-C470

Sensitivity

<10 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0950 is reactive to MMP12 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin).

Application Guarantee

EK0950 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0950-CAP Anti-Human MMP12 Pre-coated 96-well strip microplate 1
EK0950-ST Human MMP12 Standard 2 vials, 10 ng/tube
EK0950-DA Human MMP12 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0490.1360.2030.3060.5640.9651.5712.280

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human MMP-12 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)20149910291975071084
Standard deviation12.8620.4559.6813.3930.4262.87
CV (%)6.4%4.1%5.8%6.8%6%5.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of MMP-12 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 120117218119718711.776.2%
Sample 24995015115045034.540.9%
Sample 3102998596195798328.632.9%
*number of samples for each test n=16.

Gene/Protein Information For MMP12 (Source: Uniprot.Org, NCBI)

Uniprot ID

P39900

Gene ID

4321

Gene Name

MMP12

Full Name

Macrophage metalloelastase

Weight

54.002kDa

Superfamily

peptidase M10A family

Alternative Names

EC 3.4.24; hME; HMEEC 3.4.24.65; Macrophage elastase; macrophage metalloelastase; matrix metallopeptidase 12 (macrophage elastase); matrix metalloproteinase 12 (macrophage elastase); Matrix metalloproteinase-12; ME; MGC138506; MME; MMP12; MMP-12 MMP12 HME, ME, MME, MMP-12 matrix metallopeptidase 12 macrophage metalloelastase|matrix metallopeptidase 12 (macrophage elastase)|matrix metalloproteinase 12 (macrophage elastase)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MMP12, check out the MMP12 Infographic

MMP12 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MMP12: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0950 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Bchir S,Boumiza S,Ben Nasr H,Garrouch A,Kallel I,Tabka Z,Chahed K.Impact of cathepsin D activity and C224T polymorphism (rs17571) on chronic obstructive pulmonary disease: correlations with oxidative and inflammatory markers.Clin Exp Med.2021 Feb 21.doi:1
Species: Human
EK0950 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Li J,Xie Y,Zhao P,Qin Y,Oliver BG,Tian Y,Li S,Wang M,Liu X. A chinese herbal formula ameliorates COPD by inhibiting the inflammatory response via downregulation of p65, JNK, and p38. Phytomedicine. 2021 Jan 20;83:153475.doi:10.1016/j.phymed.2021.153475.Ep
Species: Human,Rat
EK0950 usage in article: APP:ELISA, SAMPLE:SERUM AND BALF, DILUTION:NA

Elastin-derived peptides are involved in the processes of human temporomandibular disorder by inducing inflammatory responses in synovial cells

Concomitant elevations of MMP?9, NGAL, proMMP?9/NGAL and neutrophil elastase in serum of smokers with chronic obstructive pulmonary disease

Narimiya T, Wada S, Kanzaki H, Ishikawa M, Tsuge A, Yamaguchi Y, Nakamura Y. J Periodontal Res. 2017 Apr 10. doi: 10.1111/jre.12453. Orthodontic tensile strain induces angiogenesis via type IV collagen degradation by matrix metalloproteinase-12.

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9 Customer Q&As for Human MMP-12 ELISA Kit PicoKine™

Question

Can EK0950 recognize the catalytic domain of MMP-12?

Verified customer

Asked: 2020-07-02

Answer

The immunogen sequence of both the capture and detection antibodies in kit Human MMP-12 ELISA Kit PicoKine™ (EK0950) was L17-C470. According to the sequence, the antibodies could recognize the catalytic domain of MMP-12.

Boster Scientific Support

Answered: 2020-07-03

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-04-24

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-04-24

Question

Are the standards of EK0950 natural material or recombinant? If it's recombinant, what kind of cell or living material is used for expression?

Verified customer

Asked: 2020-03-13

Answer

The standard of the Human MMP-12 ELISA Kit PicoKine EK0950 is recombinant and the expression system is NSO.

Boster Scientific Support

Answered: 2020-03-13

Question

Was a virus vector used in the recombination and/or expression process of EK0950? What kind?

Verified customer

Asked: 2020-03-13

Answer

There wasn't any virus vector used in the recombination and/or expression process of the Human MMP-12 ELISA Kit PicoKine EK0950.

Boster Scientific Support

Answered: 2020-03-13

Question

Do the standards of EK0950 include additive agent such as antiseptic or preservative? What is the composition of the additive agent?

Verified customer

Asked: 2020-03-13

Answer

The standard of the Human MMP-12 ELISA Kit PicoKine EK0950 contains 0.02% sodium azide. The buffer prior to lyophilization contains 0.02% sodium azide, 2% BSA, 0.2% Tris and 1% NaCl.

Boster Scientific Support

Answered: 2020-03-13

Question

What is the material name and concentration of Stop Solution for EK0950?

Verified customer

Asked: 2020-03-13

Answer

The Stop Solution for the Human MMP-12 ELISA Kit PicoKine EK0950 is 2% Sulphuric acid.

Boster Scientific Support

Answered: 2020-03-13

Question

Does EK0950 kit include hazardous substance such as azide, thimerosal, 2-ME, etc?

Verified customer

Asked: 2020-03-13

Answer

The Human MMP-12 ELISA Kit PicoKine EK0950 kit contains sodium azide (0.02%), sulphuric acid (2%) and proclin(0.2%).

Boster Scientific Support

Answered: 2020-03-13

Question

Q: What is the optimal O.D. value for MMP-12 ELISA kit? I used your MMP-12 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain MMP-12 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2020-01-07

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this MMP-12 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of MMP-12 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2020-01-07

Question

Q: can you suggest the dilution ratio of serum samples for detection of MMP-12 in Human serum? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2018-05-07

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the MMP-12 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-05-07

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