Anti-(Mouse) Nr4a2 Antibody (Center)

Product Name

Anti-(Mouse) Nr4a2 Antibody (Center)

View all Nurr1/NGFI-B beta/NR4A2 Antibodies

SKU/Catalog Number

M01592

Size

80 µl

Description

Boster Bio Anti-(Mouse) Nr4a2 Antibody (Center) (Catalog # M01592). Tested in WB, IHC-P application(s). This antibody reacts with Mouse, Rat.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-(Mouse) Nr4a2 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01592)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This mouse Nr4a2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 164-197 amino acids from the Central region of mouse Nr4a2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01592 is reactive to NR4A2 in Mouse, Rat

Applications

M01592 is guaranteed for IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

66.591kDa

Background of Nurr1/NGFI-B beta/NR4A2

This enzyme scavenge exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:2000
IHC-P: 1:25

Validation Images & Assay Conditions

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All lanes : Anti-Nr4a2 Antibody (Center) at 1:2000 dilution
Lane 1: mouse brain lysates
Lane 2: rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 67 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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Anti-Nr4a2 Antibody (Center)at 1:2000 dilution + mouse pancreas lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 67 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Click image to see more details

M01592 staining (Mouse) Nr4a2 in mouse brain sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

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M01592 staining (Mouse) Nr4a2 in mouse spleen sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Gene/Protein Information For NR4A2 (Source: Uniprot.org, NCBI)

Alternative Names

human homolog of; NGFI-B beta; NOT; NR4A2; nuclear receptor subfamily 4, group A, member 2; Nurr1; orphan nuclear receptor NR4A2; Orphan nuclear receptor NURR1; T-cell nuclear receptor NOT; TINUR; transcriptionally inducible nuclear receptor related 1; Transcriptionally-inducible nuclear receptor NR4A2 HZF-3, NOT, NURR1, RNR1, TINUR nuclear receptor subfamily 4 group A member 2 nuclear receptor subfamily 4 group A member 2|NGFI-B/nur77 beta-type transcription factor homolog|T-cell nuclear receptor NOT|immediate-early response protein NOT|intermediate-early receptor protein|nuclear receptor related 1|nur related protein-1, human homolog of|orphan nuclear receptor NR4A2|orphan nuclear receptor NURR1|transcriptionally inducible nuclear receptor related 1|transcriptionally-inducible nuclear receptor

For more info on NR4A2, check out the NR4A2 Infographic

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In this infographic, you will see the following information for NR4A2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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