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Product Info Summary
| SKU: | A14230 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MRPL20 Antibody
SKU/Catalog Number
A14230
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-MRPL20 Antibody (Catalog # A14230). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRPL20 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14230)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from C-terminal of human MRPL20.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A14230 is reactive to MRPL20 in Human, Mouse, Rat
Applications
A14230 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
17.443kDa
Background of MRPL20
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 21q.
Suzuki T., J. Biol. Chem. 276:21724-21736(2001).
Gregory S.G., Nature 441:315-321(2006).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from COLO cells, HUVEC cells and HeLa cells, using MRPL20 antibody A14230.
Protein Target Info & Infographic
Gene/Protein Information For MRPL20 (Source: Uniprot.org, NCBI)
Gene Name
MRPL20
Full Name
39S ribosomal protein L20, mitochondrial
Weight
17.443kDa
Superfamily
bacterial ribosomal protein bL20 family
Alternative Names
39S ribosomal protein L20, mitochondrial; FLJ10024; L20mt; MGC4779; MGC74465; mitochondrial ribosomal protein L20; MRP-L20 MRPL20 L20mt, MRP-L20 mitochondrial ribosomal protein L20 39S ribosomal protein L20, mitochondrial|mitochondrial large ribosomal subunit protein bL20m
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MRPL20, check out the MRPL20 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MRPL20: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MRPL20 Antibody (A14230)
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No publications found for A14230
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-MRPL20 Antibody
Question
We are currently using anti-MRPL20 antibody A14230 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
The anti-MRPL20 antibody (A14230) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-05
Question
Does A14230 anti-MRPL20 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
You can see on the product datasheet, A14230 anti-MRPL20 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-13
Question
I was wanting to use to test anti-MRPL20 antibody A14230 on mouse lymph mammary gland for research purposes, then I may be interested in using anti-MRPL20 antibody A14230 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
The products we sell, including anti-MRPL20 antibody A14230, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-26
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymph mammary gland using anti-MRPL20 antibody A14230. Let me know if you need anything else.
O. Anderson
Verified customer
Asked: 2016-03-21
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-03-21
