Anti-Myogenin (Skeletal Muscle Marker) Monoclonal Antibody

Myogenin antibody

Boster Bio Anti-Myogenin (Skeletal Muscle Marker) Monoclonal Antibody (Catalog # M03238). Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat, Dog, Cat, Pig. Cited in 1 publication(s).

Product Info Summary

SKU: M03238
Size: 100ug/vial
Reactive Species: Dog, Human, Mouse, Pig, Rat, Cat
Host: Mouse
Application: ELISA, IHC

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Product Name

Anti-Myogenin (Skeletal Muscle Marker) Monoclonal Antibody

View all Myogenin Antibodies

SKU/Catalog Number

M03238

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Myogenin (Skeletal Muscle Marker) Monoclonal Antibody (Catalog # M03238). Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat, Dog, Cat, Pig.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Myogenin (Skeletal Muscle Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03238)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: F5D

Isotype

IgG1, kappa

Immunogen

Rat myogenin peptide (aa 73-94) followed by rat myogenin recombinant fragment (aa30-224) (Epitope aa138-158)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M03238 is reactive to MYOG in Dog, Human, Mouse, Pig, Rat, Cat

Applications

M03238 is guaranteed for ELISA, IHC Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

25.037kDa

Background of Myogenin

Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif.MyoD family members share about 80% amino acid homology in their bHLH motifs.Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms' tumor.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA (For coating, order antibody without BSA)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For MYOG (Source: Uniprot.org, NCBI)

Gene Name

MYOG

Full Name

Myogenin

Weight

25.037kDa

Alternative Names

BHLHC3; bHLHc3Myogenic factor-4; myogenin; Class C basic helix-loop-helix protein 3; MYF4; myf-4; MYF4myogenin; MYOG; Myogenic factor 4; myogenin (myogenic factor 4); Myogenin MYOG MYF4, bHLHc3, myf-4 myogenin myogenin|class C basic helix-loop-helix protein 3|myogenic factor 4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MYOG, check out the MYOG Infographic

MYOG infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MYOG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M03238 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Immunohistochemical evidence of striated muscle cells within midfacial superficial musculoaponeurotic system

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Order DetailsPrice
M03238-100ug

100 μg

$468

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$468.00

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