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Product Info Summary
| SKU: | M03238-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Myogenin Monoclonal Antibody
SKU/Catalog Number
M03238-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Myogenin Monoclonal Antibody catalog # M03238-1. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Myogenin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03238-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFFH-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Myogenin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03238-1 is reactive to MYOG in Human, Mouse, Rat
Applications
M03238-1 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
71 kDa
Calculated molecular weight
25.037kDa
Background of Myogenin
Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
FC 1:40
Validation Images & Assay Conditions
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Western blot analysis of Myogenin expression in C2C12 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For MYOG (Source: Uniprot.org, NCBI)
Gene Name
MYOG
Full Name
Myogenin
Weight
25.037kDa
Alternative Names
BHLHC3; bHLHc3Myogenic factor-4; myogenin; Class C basic helix-loop-helix protein 3; MYF4; myf-4; MYF4myogenin; MYOG; Myogenic factor 4; myogenin (myogenic factor 4); Myogenin MYOG MYF4, bHLHc3, myf-4 myogenin myogenin|class C basic helix-loop-helix protein 3|myogenic factor 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MYOG, check out the MYOG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MYOG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Myogenin Monoclonal Antibody (M03238-1)
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No publications found for M03238-1
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Myogenin Monoclonal Antibody
Question
Can you help my question with product M03238-1, anti-Myogenin Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03238-1 anti-Myogenin Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-14
Question
I see that the anti-Myogenin Monoclonal antibody M03238-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-09
Question
We are currently using anti-Myogenin Monoclonal antibody M03238-1 for rat tissue, and we are well pleased with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-02
Answer
The anti-Myogenin Monoclonal antibody (M03238-1) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-02
Question
you antibody to test anti-Myogenin Monoclonal antibody M03238-1 on mouse skeletal muscle for research purposes, then I may be interested in using anti-Myogenin Monoclonal antibody M03238-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Miller
Verified customer
Asked: 2018-06-06
Answer
The products we sell, including anti-Myogenin Monoclonal antibody M03238-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-06-06
Question
Does anti-Myogenin Monoclonal antibody M03238-1 work on dog WB with skeletal muscle?
Verified Customer
Verified customer
Asked: 2018-02-14
Answer
Our lab technicians have not validated anti-Myogenin Monoclonal antibody M03238-1 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Myogenin Monoclonal antibody M03238-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog skeletal muscle in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-14
Question
Is a blocking peptide available for product anti-Myogenin Monoclonal antibody (M03238-1)?
C. Yang
Verified customer
Asked: 2016-09-19
Answer
We do provide the blocking peptide for product anti-Myogenin Monoclonal antibody (M03238-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-09-19
Question
Would M03238-1 anti-Myogenin Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
L. Brown
Verified customer
Asked: 2013-11-20
Answer
As indicated on the product datasheet, M03238-1 anti-Myogenin Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-11-20
