Anti-Myosin(Skeletal, Slow) Myh7 Antibody (Monoclonal, NOQ7.5.4D)

Product Name

Anti-Myosin(Skeletal, Slow) Myh7 Antibody (Monoclonal, NOQ7.5.4D)

View all MYH7 Antibodies

SKU/Catalog Number

MA1064

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Myosin (Skeletal, Slow) Myh7 Antibody (Monoclonal, NOQ7.5.4D) catalog # MA1064. Tested in IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Myosin(Skeletal, Slow) Myh7 Antibody (Monoclonal, NOQ7.5.4D) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1064)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

NOQ7.5.4D

Isotype

Mouse IgG1

Immunogen

Human skeletal muscle myosin purified from myofibrils.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

MA1064 is reactive to MYH7 in Human, Monkey, Mouse, Rat

Applications

MA1064 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

230 kDa

Calculated molecular weight

223.097kDa

Background of MYH7

Myosin is composed of 2 heavy chains of about 200,000 daltons each and 4 light chains of about 20,000 daltons each. Skeletal Myosin (slow),, also known as light chain 3 (MYL3), mapped to 3p. Fodor et al. (1989) found that the MYL3 gene has 7 exons, the last of which is completely untranslated 3-prime sequence.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.5-2μg/ml, Human, monkey, mouse, rat
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, monkey, mouse, rat, By Heat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Myosin using anti-Myosin antibody (MA1064).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: monkey heart tissue lysates,
Lane 2: monkey skeletal muscle tissue lysates,
Lane 3: rat heart tissue lysates,
Lane 4: rat skeletal muscle tissue lysates,
Lane 5: mouse heart tissue lysates,
Lane 6: mouse skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Myosin antigen affinity purified monoclonal antibody (Catalog # MA1064) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Myosin at approximately 230 kDa. The expected band size for Myosin is at 223 kDa.

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Figure 2. IHC analysis of Myosin using anti-Myosin antibody (MA1064).
Myosin was detected in a paraffin-embedded section of rat skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Myosin Antibody (MA1064) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.

Gene/Protein Information For MYH7 (Source: Uniprot.org, NCBI)

Alternative Names

cardiac muscle beta isoform; CMD1S; CMD1SMGC138376; CMH1; DKFZp451F047; MGC138378; MPD1; MYH7; MYHCB; MyHC-beta; myhc-slow; myopathy, distal 1; myosin heavy chain (AA 1-96); myosin, heavy chain 7, cardiac muscle, beta; myosin, heavy polypeptide 7, cardiac muscle, beta; Myosin-7; rhabdomyosarcoma antigen MU-RMS-40.7A; SPMD; SPMM MYH7 CMD1S, CMH1, MPD1, MYHCB, SPMD, SPMM myosin heavy chain 7 myosin-7|cardiac muscle myosin heavy chain 7 beta|myHC-beta|myhc-slow|myopathy, distal 1|myosin 7|myosin heavy chain beta-subunit|myosin, heavy chain 7, cardiac muscle, beta|myosin, heavy polypeptide 7, cardiac muscle, beta|rhabdomyosarcoma antigen MU-RMS-40.7A

For more info on MYH7, check out the MYH7 Infographic

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In this infographic, you will see the following information for MYH7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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