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Product Info Summary
| SKU: | A01606 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-NEK2 Antibody
SKU/Catalog Number
A01606
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-NEK2 Antibody (Catalog # A01606). Tested in ELISA, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-NEK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01606)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 275-300 of Human NEK2 protein.
Cross-reactivity
Predicted species reactivity based on epitope sequence homology: human, mouse, bovine, horse, rhesus macaque, dog, chimpanzee.
Reactive Species
A01606 is reactive to NEK2 in Mouse
Calculated molecular weight
51.8 kDa
Background of NEK2
NEK2, also called serine/threonine-protein kinase Nek2NimA-related protein kinase 2, NimA-like protein kinase 1 and HSPK 21, is a protein kinase involved in mitotic regulation that may have a role at the G2-M transition and may also play a role in meiosis. NEK2 has a nuclear localization and accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01606 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:2,000 - 1:10,000
IF Microscopy: User optimized
WB: 1:500 - 1:2,000
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kDa in size corresponding to NEK2 protein by western blotting in the appropriate cell lysate or extract. Splice variants exist for this protein that may result in the detection of lower molecular weight bands.
Validation Images & Assay Conditions
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Western Blot of Rabbit Anti-NEK2 Antibody. Lane 1: Opal Pre-stained Molecular Weight Marker . Lane 2: Jurkat cell lysate (10µg) spiked with rec. NEK2 protein (50ng). Lane 3: Jurkat cell lysate (10µg) spiked with rec. NEK2 protein (20ng). Lane 4: Jurkat cell lysate (10µg) spiked with rec. NEK2 protein (20ng). Lane 5: Jurkat cell lysate (10µg) . Primary Antibody: Anti-NEK2 at 1µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:70,000 for 30mins at RT. Block: BlockOut Buffer . Expect/Observed MW: ~76kDa rec NEK2.
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Possible model for NEK2 action.
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Western blot analysis is shown using Boster's Affinity Purified anti-NEK2 antibody to detect endogenous protein present in an unstimulated mouse A-20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~52 kDa correspond-ing to NEK2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques. Personal communication Steven Pelech, Kinexus Inc.
Specific Publications For Anti-NEK2 Antibody (A01606)
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Customer Q&As
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4 Customer Q&As for Anti-NEK2 Antibody
Question
We are currently using anti-NEK2 antibody A01606 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-01-21
Answer
The anti-NEK2 antibody (A01606) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-21
Question
you antibody to test anti-NEK2 antibody A01606 on human mammary gland for research purposes, then I may be interested in using anti-NEK2 antibody A01606 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
G. Li
Verified customer
Asked: 2018-07-03
Answer
The products we sell, including anti-NEK2 antibody A01606, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-07-03
Question
Will anti-NEK2 antibody A01606 work on feline WB with lymphoblast?
Verified Customer
Verified customer
Asked: 2018-02-09
Answer
Our lab technicians have not tested anti-NEK2 antibody A01606 on feline. You can run a BLAST between feline and the immunogen sequence of anti-NEK2 antibody A01606 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline lymphoblast in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-09
Question
My question regarding product A01606, anti-NEK2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Miller
Verified customer
Asked: 2016-01-01
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01606 anti-NEK2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-01-01
