Anti-Neurosecretory protein VGF VGF Antibody

Product Name

Anti-Neurosecretory protein VGF VGF Antibody

View all VGF Antibodies

SKU/Catalog Number

A03966

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Neurosecretory protein VGF VGF Antibody (Catalog # A03966). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

VGF antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Neurosecretory protein VGF VGF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03966)

Host

Rabbit

Contents

VGF Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

VGF antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of the human VGF. The immunogen is located within the last 50 amino acids of VGF.

Reactive Species

A03966 is reactive to VGF in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

67.3 kDa

Background of VGF

VGF was initially identified as a rapidly regulated gene product in nerve growth factor-treated PC12 cells. This protein is synthesized in neurons in the central and peripheral nervous system as well as in the pituitary, adrenal medulla, endocrine cells of the stomach, and pancreatic beta cells. VGF is thought to be involved in organism energy balance and regulation of homeostasis as mice lacking this gene show deficits in these areas. More recent results suggest that VGF is upregulated by brain-derived neurotrophic factor (BDNF) and can stimulate the proliferation of hippocampal progenitor cells and produce antidepressant-like behavioral effects, suggesting that this pathway may be a suitable target for therapeutic treatments.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03966 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

VGF antibody can be used for detection of VGF by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information VGF

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