Product Info Summary
| SKU: | A05691 |
|---|---|
| Size: | 100 µg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NFKBIL1 Antibody
SKU/Catalog Number
A05691
Size
100 µg
Form
Liquid
Description
Boster Bio Anti-NFKBIL1 Antibody (Catalog# A05691). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Cite This Product
Anti-NFKBIL1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05691)
Host
Rabbit
Contents
100 µg in 200 µl PBS containing 0.05% stabilizing protein and 0.05% sodium azide
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A partial length recombinant NFKBIL1 protein (amino acids 150-360)
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05691 is reactive to NFKBIL1 in Human
Calculated molecular weight
43.2 kDa
Background of NFKBIL1
SmarcAL1 (SWI/SNF-related µatrix-associated actin-dependent regulator of chromatin a-like 1) is also known as HARP, HepA Related Protein, HHARP, SMARCA like Protein 1 and SWI/SNF Related. SmarcAL1 is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05691 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot: 2-4 µg/ml
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of NFKBIL1. Anti-NFKBIL1 antibody was used at 4 &mu
Specific Publications For Anti-NFKBIL1 Antibody (A05691)
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Customer Q&As
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3 Customer Q&As for Anti-NFKBIL1 Antibody
Question
I was wanting to use your anti-NFKBIL1 antibody for WB for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
D. Krishna
Verified customer
Asked: 2016-07-25
Answer
You can see on the product datasheet, A05691 anti-NFKBIL1 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-07-25
Question
We are currently using anti-NFKBIL1 antibody A05691 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
A. Krishna
Verified customer
Asked: 2016-03-21
Answer
The anti-NFKBIL1 antibody (A05691) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-03-21
Question
I see that the anti-NFKBIL1 antibody A05691 works with WB, what is the protocol used to produce the result images on the product page?
O. Bhatt
Verified customer
Asked: 2014-03-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2014-03-06

