Product Info Summary
SKU: | A03724-1 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-NUP107 Antibody Picoband™
View all Nucleoporin 107 Antibodies
SKU/Catalog Number
A03724-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-NUP107 Antibody Picoband™ catalog # A03724-1. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NUP107 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03724-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NUP107 recombinant protein (Position: K30-H790).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03724-1 is reactive to NUP107 in Human, Mouse, Rat
Applications
A03724-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
106 kDa
Calculated molecular weight
106.374kDa
Background of Nucleoporin 107
Nucleoporin 107 (Nup107) is a protein that in humans is encoded by the NUP107 gene. This gene encodes a member of the nucleoporin family. The protein is localized to the nuclear rim and is an essential component of the nuclear pore complex (NPC). All molecules entering or leaving the nucleus either diffuse through or are actively transported by the NPC. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of NUP107 using anti-NUP107 antibody (A03724-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Colo320 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: human PC-3 whole cell lysates,
Lane 6: human K562 whole cell lysates,
Lane 7: human HEL whole cell lysates,
Lane 8: rat PC-12 whole cell lysates,
Lane 9: mouse testis tissue lysates,
Lane 10: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP107 antigen affinity purified polyclonal antibody (Catalog # A03724-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUP107 at approximately 106 kDa. The expected band size for NUP107 is at 106 kDa.
Click image to see more details
Figure 2. IF analysis of NUP107 using anti-NUP107 antibody (A03724-1).
NUP107 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NUP107 Antibody (A03724-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The tissue section was developed using Phalloidin-iFluor 594 Conjugated. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 3. Flow Cytometry analysis of HEL cells using anti-NUP107 antibody (A03724-1).
Overlay histogram showing HEL cells stained with A03724-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NUP107 Antibody (A03724-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For NUP107 (Source: Uniprot.org, NCBI)
Gene Name
NUP107
Full Name
Nuclear pore complex protein Nup107
Weight
106.374kDa
Superfamily
nucleoporin Nup84/Nup107 family
Alternative Names
nuclear pore complex protein Nup107; nucleoporin 107kDa; NUP84Nucleoporin Nup107,107 kDa nucleoporin NUP107 NPHS11, NUP84, ODG6, ODG6; GAMOS7 nucleoporin 107 nuclear pore complex protein Nup107|nucleoporin 107kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NUP107, check out the NUP107 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NUP107: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NUP107 Antibody Picoband™ (A03724-1)
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No publications found for A03724-1
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