Anti-NUP35 Antibody

Nup53 antibody

Boster Bio Anti-NUP35 Antibody (Catalog# A08614). Tested in WB application(s). This antibody reacts with Human.

Product Info Summary

SKU: A08614
Size: 100 µg
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-NUP35 Antibody

View all Nup53 Antibodies

SKU/Catalog Number

A08614

Size

100 µg

Form

Liquid

Description

Boster Bio Anti-NUP35 Antibody (Catalog# A08614). Tested in WB application(s). This antibody reacts with Human.

Storage & Handling

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Cite This Product

Anti-NUP35 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08614)

Host

Rabbit

Contents

100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A partial length recombinant NUP35 protein (amino acids 26-241)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A08614 is reactive to Nup35 in Human

Applications

A08614 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

34.786kDa

Background of Nup53

SmarcAL1 (SWI/SNF-related µatrix-associated actin-dependent regulator of chromatin a-like 1) is also known as HARP, HepA Related Protein, HHARP, SMARCA like Protein 1 and SWI/SNF Related. SmarcAL1 is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot: 1-2 µg/ml

Validation Images & Assay Conditions

Gene/Protein Information For Nup35 (Source: Uniprot.org, NCBI)

Gene Name

Nup35

Full Name

Nucleoporin NUP35

Weight

34.786kDa

Superfamily

Nup35 family

Alternative Names

35 kDa nucleoporin; Mitotic phosphoprotein 44; MP-44; MP44NUP53; NP44; Nuclear pore complex protein Nup53; nucleoporin 35kDa; Nucleoporin Nup35; nucleoporin NUP53 Nup35|2310006I24Rik, 35kDa, 5330402E05Rik, MP44, NO44|nucleoporin 35|nucleoporin NUP35|35 kDa nucleoporin|mitotic phosphoprotein 44|nuclear pore complex protein Nup53

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Nup35, check out the Nup35 Infographic

Nup35 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Nup35: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-NUP35 Antibody

Question

We want to test anti-NUP35 antibody A08614 on human oocyte for research purposes, then I may be interested in using anti-NUP35 antibody A08614 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-10-25

Answer

The products we sell, including anti-NUP35 antibody A08614, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-10-25

Question

Is there a BSA free version of anti-NUP35 antibody A08614 available?

Verified Customer

Verified customer

Asked: 2019-10-09

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NUP35 antibody A08614 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-10-09

Question

Does anti-NUP35 antibody A08614 work for WB with oocyte?

C. Carter

Verified customer

Asked: 2018-10-26

Answer

According to the expression profile of oocyte, NUP35 is highly expressed in oocyte. So, it is likely that anti-NUP35 antibody A08614 will work for WB with oocyte.

Boster Scientific Support

Answered: 2018-10-26

Question

We are currently using anti-NUP35 antibody A08614 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2018-09-18

Answer

The anti-NUP35 antibody (A08614) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-09-18

Question

Here is the WB image, lot number and protocol we used for oocyte using anti-NUP35 antibody A08614. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-07-24

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-07-24

Question

Can you help my question with product A08614, anti-NUP35 antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

S. Singh

Verified customer

Asked: 2014-11-24

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08614 anti-NUP35 antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-11-24

Order DetailsPrice
A08614-100ug

100 μg

$341

Free Secondary Antibody

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A08614
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$341.00

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