Product Info Summary
SKU: | A08614 |
---|---|
Size: | 100 µg |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-NUP35 Antibody
SKU/Catalog Number
A08614
Size
100 µg
Form
Liquid
Description
Boster Bio Anti-NUP35 Antibody (Catalog# A08614). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Cite This Product
Anti-NUP35 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08614)
Host
Rabbit
Contents
100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A partial length recombinant NUP35 protein (amino acids 26-241)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A08614 is reactive to Nup35 in Human
Applications
A08614 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
34.786kDa
Background of Nup53
SmarcAL1 (SWI/SNF-related µatrix-associated actin-dependent regulator of chromatin a-like 1) is also known as HARP, HepA Related Protein, HHARP, SMARCA like Protein 1 and SWI/SNF Related. SmarcAL1 is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot: 1-2 µg/ml
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of NUP35. Anti-NUP35 antibody was used at 1 &mu
Protein Target Info & Infographic
Gene/Protein Information For Nup35 (Source: Uniprot.org, NCBI)
Gene Name
Nup35
Full Name
Nucleoporin NUP35
Weight
34.786kDa
Superfamily
Nup35 family
Alternative Names
35 kDa nucleoporin; Mitotic phosphoprotein 44; MP-44; MP44NUP53; NP44; Nuclear pore complex protein Nup53; nucleoporin 35kDa; Nucleoporin Nup35; nucleoporin NUP53 Nup35|2310006I24Rik, 35kDa, 5330402E05Rik, MP44, NO44|nucleoporin 35|nucleoporin NUP35|35 kDa nucleoporin|mitotic phosphoprotein 44|nuclear pore complex protein Nup53
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Nup35, check out the Nup35 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Nup35: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NUP35 Antibody (A08614)
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No publications found for A08614
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-NUP35 Antibody
Question
We want to test anti-NUP35 antibody A08614 on human oocyte for research purposes, then I may be interested in using anti-NUP35 antibody A08614 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
The products we sell, including anti-NUP35 antibody A08614, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-25
Question
Is there a BSA free version of anti-NUP35 antibody A08614 available?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NUP35 antibody A08614 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-09
Question
Does anti-NUP35 antibody A08614 work for WB with oocyte?
C. Carter
Verified customer
Asked: 2018-10-26
Answer
According to the expression profile of oocyte, NUP35 is highly expressed in oocyte. So, it is likely that anti-NUP35 antibody A08614 will work for WB with oocyte.
Boster Scientific Support
Answered: 2018-10-26
Question
We are currently using anti-NUP35 antibody A08614 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-09-18
Answer
The anti-NUP35 antibody (A08614) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-18
Question
Here is the WB image, lot number and protocol we used for oocyte using anti-NUP35 antibody A08614. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-07-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-24
Question
Can you help my question with product A08614, anti-NUP35 antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Singh
Verified customer
Asked: 2014-11-24
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08614 anti-NUP35 antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-11-24