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Product Info Summary
| SKU: | M14439 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-OPAL1 Purified WBP1L Monoclonal Antibody
SKU/Catalog Number
M14439
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-OPAL1 Purified WBP1L Monoclonal Antibody (Catalog# M14439). Tested in Flow Cytometry, WB application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-OPAL1 Purified WBP1L Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M14439)
Host
Mouse
Contents
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
OPAL1-01
Isotype
Mouse IgG2a
Immunogen
Recombinant fragment of human OPAL1 (amino acids 152-342). The mouse monoclonal antibody OPAL1-01 recognizes an intracellular epitope of OPAL1 (outcome predictor in acute leukemia 1), a transmembrane adaptor protein expressed mainly by megacaryocytes and platelets.
Cross-reactivity
Following cross-reactivity (%) was determined by classic double-antibody RIA using unlabelled hormones: beta-hCG (77), alpha-hCG (1.3), hLH (0.86), hFSH (< 0.5), hTSH (< 0.5).
Reactive Species
M14439 is reactive to WBP1L in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
37.8 kDa
Background of WBP1L
OPAL1 (outcome predictor in acute leukemia 1) is an almost uncharacterized transmembrane adaptor protein expressed mainly by megacaryocytes and platelets. Its expression is enhanced on TEL/AML leukemic cells. The function of OPAL1 is unknown, although the presence of a cytochrome c-like heme-binding site and a transmembrane domain suggested OPAL1 may be involved in the mitochondrial electron transport chain. Its originally reported prognostic impact appears to be treatment dependent.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M14439 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 µg/ml, intracellular staining.
Western blotting: 1-2 μg/ml, non-reducing conditions.
Validation Images & Assay Conditions
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Flow cytometry analysis of OPAL-transfected HEK293 cells using mouse monoclonal antibody OPAL1-01.
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Western blotting analysis of human OPAL1 using mouse monoclonal antibody OPAL1-01 on lysates of HEK-293T/OPAL1 transfectants, and of murine splenocytes and HEK-293T cells (negative controls) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-OPAL1 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. OPAL1 was detected at around 45-60 kDa.
Specific Publications For Anti-OPAL1 Purified WBP1L Monoclonal Antibody (M14439)
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