Anti-ORC1 Antibody

ORC1 antibody

Boster Bio Anti-ORC1 Antibody catalog # A02735. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A02735
Size: 100μl
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-ORC1 Antibody

View all ORC1 Antibodies

SKU/Catalog Number

A02735

Size

100μl

Form

Liquid

Description

Boster Bio Anti-ORC1 Antibody catalog # A02735. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ORC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02735)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

4H4

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human ORC1. at AA rangle: 300-380

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02735 is reactive to ORC1 in Human

Applications

A02735 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

97.35kDa

Background of ORC1

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of a-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Emamian ES, et al. (2004) J Neurosci. 24(7): 1561-4
Palmer, C.L. et al. (2005) Pharmacol. Rev. 57, 253-277.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For ORC1 (Source: Uniprot.org, NCBI)

Gene Name

ORC1

Full Name

Origin recognition complex subunit 1

Weight

97.35kDa

Superfamily

ORC1 family

Alternative Names

HS; HSORC1; ORC1Lorigin recognition complex subunit 1; origin recognition complex, subunit 1 (yeast homolog)-like; origin recognition complex, subunit 1 homolog (S. cerevisiae); origin recognition complex, subunit 1 homolog; origin recognition complex, subunit 1; origin recognition complex, subunit 1-like (yeast); origin recognition complex, subunit 1-like; PARC1origin recognition complex 1; Replication control protein 1 ORC1 HSORC1L, PARC1, ORC1 origin recognition complex subunit 1 origin recognition complex subunit 1|origin recognition complex, subunit 1 homolog|replication control protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ORC1, check out the ORC1 Infographic

ORC1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ORC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-ORC1 Antibody

Question

Does A02735 anti-ORC1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

R. Jones

Verified customer

Asked: 2019-07-30

Answer

You can see on the product datasheet, A02735 anti-ORC1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-07-30

Question

I see that the anti-ORC1 antibody A02735 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-06-28

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-28

Question

We are currently using anti-ORC1 antibody A02735 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-05-22

Answer

The anti-ORC1 antibody (A02735) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-22

Question

See attached the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-ORC1 antibody A02735. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-12-21

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-12-21

Question

Is a blocking peptide available for product anti-ORC1 antibody (A02735)?

S. Krishna

Verified customer

Asked: 2014-12-23

Answer

We do provide the blocking peptide for product anti-ORC1 antibody (A02735). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2014-12-23

Order DetailsPrice
A02735

100uL

$399

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A02735
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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