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Product Info Summary
| SKU: | A02118 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, IP, IHC, WB |
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Product info
Product Name
Anti-p130 Rb2 RBL2 Antibody
SKU/Catalog Number
A02118
Size
100uL
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-p130 Rb2 RBL2 Antibody (Catalog # A02118). Tested in IHC, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-p130 Rb2 RBL2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02118)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
Rb2 (p130) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A02118 is reactive to RBL2 in Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
128.4 kDa
Background of RBL2
Retinoblastoma-like protein 2 (Rb2) is a key regulator of entry into cell division. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. p130 recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. It controls histone H4 'Lys-20' trimethylation and probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. It is a potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. It binds to cyclins A and E as well as binds to and may be involved in the transforming capacity of the adenovirus E1A protein. Rb2 may act as a tumor suppressor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02118 is guaranteed for ELISA, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000 - 1:20,000
IHC: 1:200 - 1:1,000
IP: 1:100
WB: 1:500 - 1:2,000
Anti-p130 has been tested by western blot and immunohistochemistry and is suitable for ELISA, immunoprecipitation, immunoblotting, immunohistochemistry, and other immunological methods requiring high titer and specificity.
Validation Images & Assay Conditions
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Immunohistochemical staining of mouse tissue using anti-pRb2/p130 antiserum. The staining shows the location of pRb2/p130 in developing mouse tissue. Other detection systems should yield similar results. Sections were cut at 5-7 µm, mounted on glass and dried overnight at 37°C. All sections were deparaffinized in xylene, rehydrated through a graded alcohol series and washed in phosphate-buffered saline (PBS). PBS was used for all subsequent washes and for antiserum dilution. Tissue sections were quenched sequentially in 0.5% hydrogen peroxide and blocked with diluted 10% normal goat anti-rabbit serum. Slides were incubated at 20° C for 1 h with rabbit anti-pRb2/p130 (1:500) dilution, washed, and then reacted with diluted goat anti-rabbit biotinylated antibody for 30 min. Slides were then reacted with streptavidin-peroxidase conjugate for 30 min at 20° C. Diaminobenzidine was used as the final chromogen. Negative controls for each tissue section were prepared by substituting the primary antiserum with pre-immune serum.
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Western Blot of Rabbit Anti-Rb2 p130 Antibody. Lane 1: HEK 293 pcDNA3. Lane 2: HEK 293 pcDNA3-Rb2wt. Lane 3: HEK 293 pcDNA3-Rb2-PM19. Load: 30 µg per lane. Primary antibody: Anti-Rb2 antibody at 1:250 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 130 kDa for p130/Rb2.
Specific Publications For Anti-p130 Rb2 RBL2 Antibody (A02118)
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