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Product Info Summary
| SKU: | P30452 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC |
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Product info
Product Name
Anti-Phospho NIFK (Thr234) Antibody
SKU/Catalog Number
P30452
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho NIFK (Thr234) Antibody (Catalog# P30452). Tested in IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho NIFK (Thr234) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P30452)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
BEF-20
Isotype
Rabbit IgG
Immunogen
A peptide derived from human NIFK around the phosphorylation site of Thr234. Immunogen sequence location: 200-249
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P30452 is reactive to Nifk in Human, Mouse
Applications
P30452 is guaranteed for ELISA, IF, IHC-P, ICC Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
36.265kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human brain, using NIFK (Phospho-Thr234) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunofluorescence analysis of HUVEC cells, using NIFK (Phospho-Thr234) Antibody. The picture on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For Nifk (Source: Uniprot.org, NCBI)
Gene Name
Nifk
Full Name
MKI67 FHA domain-interacting nucleolar phosphoprotein
Weight
36.265kDa
Alternative Names
MKI67 FHA domain-interacting nucleolar phosphoprotein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Nifk, check out the Nifk Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Nifk: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho NIFK (Thr234) Antibody (P30452)
Hello CJ!
No publications found for P30452
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Phospho NIFK (Thr234) Antibody
Question
We are currently using anti-Phospho NIFK (Thr234) antibody P30452 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on primate tissues as well?
S. Thomas
Verified customer
Asked: 2016-05-30
Answer
The anti-Phospho NIFK (Thr234) antibody (P30452) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-05-30
Question
I see that the anti-Phospho NIFK (Thr234) antibody P30452 works with IF, what is the protocol used to produce the result images on the product page?
R. Wu
Verified customer
Asked: 2014-09-04
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-09-04
Question
Does anti-Phospho NIFK (Thr234) antibody P30452 work for IF with cervix carcinoma?
E. Dhar
Verified customer
Asked: 2013-06-14
Answer
According to the expression profile of cervix carcinoma, NIFK is highly expressed in cervix carcinoma. So, it is likely that anti-Phospho NIFK (Thr234) antibody P30452 will work for IF with cervix carcinoma.
Boster Scientific Support
Answered: 2013-06-14
