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Product Info Summary
| SKU: | A02293 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-m7GpppN-mRNA hydrolase DCP2 Antibody
SKU/Catalog Number
A02293
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-m7GpppN-mRNA hydrolase DCP2 Antibody (Catalog # A02293). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
DCP2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-m7GpppN-mRNA hydrolase DCP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02293)
Host
Rabbit
Contents
DCP2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
DCP2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human DCP2. The immunogen is located within amino acids 340 - 390 of DCP2.
Cross-reactivity
Multiple isoforms of DCP2 are known to exist.
Reactive Species
A02293 is reactive to DCP2 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
48.4 kDa
Background of DCP2
The removal, or decapping, of eukaryotic mRNA is an important step in the degradation of mRNA. Decapping protein 2 (DCP2) is the major mRNA decapping enzyme in cells. It is a member of the Nudix hydrolases superfamily of proteins that predominantly catalyze the hydrolysis of small nucleoside diphosphate substrates linked to another moiety. DCP2 is widely expressed in multiple tissues at varying levels, with highest expression seen in testis and brain.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02293 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
DCP2 antibody can be used for detection of DCP2 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of DCP2 in 293 cell lysate with DCP2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunofluorescence of DCP2 in human testis tissue with DCP2 antibody at 20 μg/mL.
Specific Publications For Anti-m7GpppN-mRNA hydrolase DCP2 Antibody (A02293)
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