Anti-Phospho-Thr356 PRAS40 Antibody, Phospho-Specific

PRAS40 antibody

Boster Bio Anti-Phospho-Thr356 PRAS40 Antibody, Phospho-Specific (Catalog # P03629). Tested in WB applications. This antibody reacts with Drosophila.

Product Info Summary

SKU: P03629
Size: 100 µl
Reactive Species: Drosophila
Host: Rabbit
Application: WB

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Product Name

Anti-Phospho-Thr356 PRAS40 Antibody, Phospho-Specific

View all PRAS40 Antibodies

SKU/Catalog Number

P03629

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-Thr356 PRAS40 Antibody, Phospho-Specific (Catalog # P03629). Tested in WB applications. This antibody reacts with Drosophila.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-Thr356 PRAS40 Antibody, Phospho-Specific (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03629)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr356 of Drosophila PRAS40, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Drosophila.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P03629 is reactive to Akt1s1 in Drosophila

Applications

P03629 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

27.483kDa

Background of PRAS40

PRAS40 (proline-rich Akt/PKB substrate of 40 kDa) acts at the intersection of the Akt- and mTOR-mediated signaling pathways and its phosphorylation by Akt and by mTORC1 results in dissociation of PRAS40 from mTORC1 which may relieve an inhibitory constraint on mTORC1 activity (Wiza et al., 2012). Phosphorylation of PRAS40 by Akt and association with 14-3-3 has also been indicated to be crucial for insulin to stimulate mTOR. (Vander Haar et al., 2007). The primary function of PRAS40 in vivo in Drosophila has been shown to regulate TORC1 activity, and not to act as a downstream target and effector of TORC1 (Pallares et al., 2012).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

Gene/Protein Information For Akt1s1 (Source: Uniprot.org, NCBI)

Gene Name

Akt1s1

Full Name

Proline-rich AKT1 substrate 1

Weight

27.483kDa

Alternative Names

40 kDa; AKT1 substrate 1 (proline-rich); AKT1S1; Lobe; MGC2865; PRAS40; proline-rich AKT1 substrate 1,40 kDa proline-rich AKT substrate Akt1s1|1110012J22Rik, AI227026, Lo, Lobe, Lobel, PR, PRAS40|AKT1 substrate 1 (proline-rich)|proline-rich AKT1 substrate 1|proline-rich AKT substrate

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Akt1s1, check out the Akt1s1 Infographic

Akt1s1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Akt1s1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-Phospho-Thr356 PRAS40 Antibody, Phospho-Specific

Question

Will anti-Phospho-Thr356 PRAS40 antibody, Phospho-Specific P03629 work on bovine WB with brain {eco:0000312?

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

Our lab technicians have not validated anti-Phospho-Thr356 PRAS40 antibody, Phospho-Specific P03629 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Phospho-Thr356 PRAS40 antibody, Phospho-Specific P03629 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine brain {eco:0000312 in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-23

Question

Will P03629 anti-Phospho-Thr356 PRAS40 antibody, Phospho-Specific work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-10-04

Answer

As indicated on the product datasheet, P03629 anti-Phospho-Thr356 PRAS40 antibody, Phospho-Specific as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-04

Question

I see that the anti-Phospho-Thr356 PRAS40 antibody, Phospho-Specific P03629 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-08-15

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-08-15

Question

Does anti-Phospho-Thr356 PRAS40 antibody, Phospho-Specific P03629 work for WB with colon carcinoma?

Verified Customer

Verified customer

Asked: 2018-10-26

Answer

According to the expression profile of colon carcinoma, AKT1S1 is highly expressed in colon carcinoma. So, it is likely that anti-Phospho-Thr356 PRAS40 antibody, Phospho-Specific P03629 will work for WB with colon carcinoma.

Boster Scientific Support

Answered: 2018-10-26

Question

We are currently using anti-Phospho-Thr356 PRAS40 antibody, Phospho-Specific P03629 for drosophila tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says drosophila. Is it likely that the antibody can work on zebrafish tissues as well?

M. Krishna

Verified customer

Asked: 2016-01-08

Answer

The anti-Phospho-Thr356 PRAS40 antibody, Phospho-Specific (P03629) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-01-08

Question

I was wanting to use your anti-Phospho-Thr356 PRAS40 antibody, Phospho-Specific for WB for drosophila colon carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for drosophila colon carcinoma identification?

J. Collins

Verified customer

Asked: 2014-03-21

Answer

It shows on the product datasheet, P03629 anti-Phospho-Thr356 PRAS40 antibody, Phospho-Specific has been tested for WB on drosophila tissues. We have an innovator award program that if you test this antibody and show it works in drosophila colon carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-03-21

Size

Total: $465

SKU:P03629

Backordered.

Lead time for this item is typically 6-8 days

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P03629
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$465.00

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