Product Info Summary
SKU: | M03629 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IHC, WB |
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Product info
Product Name
Anti-PRAS40 AKT1S1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M03629
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PRAS40 AKT1S1 Rabbit Monoclonal Antibody catalog # M03629. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PRAS40 AKT1S1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03629)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABIO-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PRAS40
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M03629 is reactive to Akt1s1 in Human, Mouse
Applications
M03629 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee
Observed Molecular Weight
58 kDa
Calculated molecular weight
27.483kDa
Background of PRAS40
Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of PRAS40 expression in 293T cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For Akt1s1 (Source: Uniprot.org, NCBI)
Gene Name
Akt1s1
Full Name
Proline-rich AKT1 substrate 1
Weight
27.483kDa
Alternative Names
40 kDa; AKT1 substrate 1 (proline-rich); AKT1S1; Lobe; MGC2865; PRAS40; proline-rich AKT1 substrate 1,40 kDa proline-rich AKT substrate Akt1s1|1110012J22Rik, AI227026, Lo, Lobe, Lobel, PR, PRAS40|AKT1 substrate 1 (proline-rich)|proline-rich AKT1 substrate 1|proline-rich AKT substrate
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Akt1s1, check out the Akt1s1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Akt1s1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PRAS40 AKT1S1 Rabbit Monoclonal Antibody (M03629)
Hello CJ!
No publications found for M03629
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-PRAS40 AKT1S1 Rabbit Monoclonal Antibody
Question
Has M03629 and M03629-1 been tested for Co-IP, immunocytochemistry and immunohistochemistry? What is the immunogen sequence?
Verified customer
Asked: 2019-04-19
Answer
Yes, the Anti-PRAS40 AKT1S1 Rabbit Monoclonal Antibody (M03629) and Anti-PRAS40 AKT1S1 Monoclonal Antibody (M03629-1) have been tested for immunocytochemistry.
Boster Scientific Support
Answered: 2019-04-22