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Product Info Summary
| SKU: | M03629-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-PRAS40 AKT1S1 Monoclonal Antibody
SKU/Catalog Number
M03629-1
BM5325 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PRAS40 AKT1S1 Monoclonal Antibody catalog # M03629-1. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PRAS40 AKT1S1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03629-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACHD-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PRAS40 Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt. One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 protein. PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex.
Reactive Species
M03629-1 is reactive to AKT1S1 in Human, Mouse, Rat
Observed Molecular Weight
40 kDa
Calculated molecular weight
27.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03629-1 is guaranteed for IP, WB Boster Guarantee
Recommend Dilution
WB 1:500-2000
IP 1:20
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Western blot analysis of PRAS40 expression in (1) HeLa cell lysate; (2) RAW 264.7 cell lysate; (3) PC12 cell lysate.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
Specific Publications For Anti-PRAS40 AKT1S1 Monoclonal Antibody (M03629-1)
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Customer Q&As
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2 Customer Q&As for Anti-PRAS40 AKT1S1 Monoclonal Antibody
Question
Has M03629 and M03629-1 been tested for Co-IP, immunocytochemistry and immunohistochemistry? What is the immunogen sequence?
Verified customer
Asked: 2019-04-19
Answer
Yes, the Anti-PRAS40 AKT1S1 Rabbit Monoclonal Antibody (M03629) and Anti-PRAS40 AKT1S1 Monoclonal Antibody (M03629-1) have been tested for immunocytochemistry.
Boster Scientific Support
Answered: 2019-04-22
Question
What are the samples used for the WB test for M03629-1?
Verified customer
Asked: 2019-04-19
Answer
The samples tested for Anti-PRAS40 AKT1S1 Monoclonal Antibody (M03629-1) are; (1) HeLa cell lysate (2) RAW 264.7 cell lysate (3) PC12 cell lysate
Boster Scientific Support
Answered: 2019-04-22
