Anti-Proline-rich AKT1 substrate 1 AKT1S1 Antibody

Product Name

Anti-Proline-rich AKT1 substrate 1 AKT1S1 Antibody

View all AKT1S1 Antibodies

SKU/Catalog Number

A03629

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Proline-rich AKT1 substrate 1 AKT1S1 Antibody (Catalog # A03629). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

AKT1S1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-Proline-rich AKT1 substrate 1 AKT1S1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03629)

Host

Rabbit

Contents

AKT1S1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

AKT1S1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human AKT1S1. The immunogen is located within amino acids 190 - 240 of AKT1S1.

Cross-reactivity

AKT1S1 antibody is human, mouse and rat reactive. At least three isoforms of AKT1S1 are known to exist.

Reactive Species

A03629 is reactive to AKT1S1 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

27.4 kDa

Background of AKT1S1

The Akt signaling pathway contributes to the regulation of apoptosis after a variety of cell death signals. AKT1S1, also known as PRAS40, is a proline-rich substrate of the kinase AKT1 and is thought to play a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia (1). AKT1S1 is also a substrate and potential regulator of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy (2). Treatment with the insulin-like growth factor-1 (IGF1) can indusce the phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells (3).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03629 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

AKT1S1 antibody can be used for detection of AKT1S1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information AKT1S1

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