Product Info Summary
SKU: | A03629 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Proline-rich AKT1 substrate 1 AKT1S1 Antibody
SKU/Catalog Number
A03629
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Proline-rich AKT1 substrate 1 AKT1S1 Antibody (Catalog # A03629). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
AKT1S1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Proline-rich AKT1 substrate 1 AKT1S1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03629)
Host
Rabbit
Contents
AKT1S1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
SK7
Isotype
IgG
Immunogen
AKT1S1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human AKT1S1. The immunogen is located within amino acids 190 - 240 of AKT1S1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
AKT1S1 antibody is human, mouse and rat reactive. At least three isoforms of AKT1S1 are known to exist.
Reactive Species
A03629 is reactive to Akt1s1 in Human, Mouse, Rat
Applications
A03629 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
27.483kDa
Background of PRAS40
The Akt signaling pathway contributes to the regulation of apoptosis after a variety of cell death signals. AKT1S1, also known as PRAS40, is a proline-rich substrate of the kinase AKT1 and is thought to play a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia (1). AKT1S1 is also a substrate and potential regulator of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy (2). Treatment with the insulin-like growth factor-1 (IGF1) can indusce the phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
AKT1S1 antibody can be used for detection of AKT1S1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of AKT1S1 in human brain tissue lysate with AKT1S1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of AKT1S1 in rat brain tissue with AKT1S1 antibody at 2.5 μg/ml.
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Immunofluorescence of AKT1S1 in rat brain tissue with AKT1S1 antibody at 20 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For Akt1s1 (Source: Uniprot.org, NCBI)
Gene Name
Akt1s1
Full Name
Proline-rich AKT1 substrate 1
Weight
27.483kDa
Alternative Names
40 kDa; AKT1 substrate 1 (proline-rich); AKT1S1; Lobe; MGC2865; PRAS40; proline-rich AKT1 substrate 1,40 kDa proline-rich AKT substrate Akt1s1|1110012J22Rik, AI227026, Lo, Lobe, Lobel, PR, PRAS40|AKT1 substrate 1 (proline-rich)|proline-rich AKT1 substrate 1|proline-rich AKT substrate
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Akt1s1, check out the Akt1s1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Akt1s1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Proline-rich AKT1 substrate 1 AKT1S1 Antibody (A03629)
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No publications found for A03629
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