Anti-Piccolo Monoclonal Antibody

Product Name

Anti-Piccolo Monoclonal Antibody

View all Piccolo Antibodies

SKU/Catalog Number

M05881

Size

100ug

Form

liquid

Description

Boster Bio Anti-Piccolo Monoclonal Antibody catalog # M05881. Tested in ELISA, IF, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Piccolo Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05881)

Host

Mouse

Contents

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Clonality

Monoclonal

Clone Number

6H9-B6

Isotype

IgG2b Kappa

Immunogen

Human full length recombinant protein (GenBank ID: BC001304.1)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~550kDa. No cross-reactivity to Bassoon.

Reactive Species

M05881 is reactive to Pclo in Human, Mouse, Rat

Applications

M05881 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

550.834kDa

Background of Piccolo

Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains (1, 2). In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones) (3). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1 (2).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Validation Images & Assay Conditions

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Figure 2. Western blot analysis of Pclo using anti-Pclo antibody (M05881).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pclo antigen affinity purified polyclonal antibody (Catalog # M05881) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for Pclo.

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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Piccolo Monoclonal Antibody, Clone 6H9-B6 (M05881) . Tissue: Neuroblastoma cell line (SK-N-BE) . Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Piccolo Monoclonal Antibody (M05881) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Piccolo Antibody. (D) Composite.

Gene/Protein Information For Pclo (Source: Uniprot.org, NCBI)

Alternative Names

ACZ; Aczonin; PCLO; piccolo (presynaptic cytomatrix protein); Piccolo; protein piccolo Pclo|Ac, Acz, P, Pico|piccolo (presynaptic cytomatrix protein)|protein piccolo|aczonin|brain-derived HLMN protein|multidomain presynaptic cytomatrix protein

For more info on Pclo, check out the Pclo Infographic

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In this infographic, you will see the following information for Pclo: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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