Anti-PID1 Antibody Picoband™

Product Name

Anti-PID1 Antibody Picoband™

View all PID1 Antibodies

SKU/Catalog Number

A07451-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PID1 Antibody Picoband™ catalog # A07451-1. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PID1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07451-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human PID1 recombinant protein (Position: M1-K227). Human PID1 shares 97.3% amino acid (aa) sequence identity with mouse PID1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07451-1 is reactive to PID1 in Human, Mouse, Rat

Applications

A07451-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

28 kDa

Calculated molecular weight

28.272kDa

Background of PID1

This gene can increase proliferation of preadipocytes without affecting adipocytic differentiation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PID1 using anti-PID1 antibody (A07451-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human SIHA whole cell lysates,
Lane 3: rat liver tissue lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat RH35 whole cell lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PID1 antigen affinity purified polyclonal antibody (Catalog # A07451-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PID1 at approximately 28 kDa. The expected band size for PID1 is at 28 kDa.

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Figure 2. Flow Cytometry analysis of U251 cells using anti-PID1 antibody (A07451-1).
Overlay histogram showing U251 cells stained with A07451-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PID1 Antibody (A07451-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For PID1 (Source: Uniprot.org, NCBI)

Alternative Names

PTB-containing, cubilin and LRP1-interacting protein PID1 HMFN2073, NYGGF4, P-CLI1, PCLI1 phosphotyrosine interaction domain containing 1 PTB-containing, cubilin and LRP1-interacting protein|phosphotyrosine interaction domain-containing protein 1

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In this infographic, you will see the following information for PID1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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