Anti-PIWI-L1 Antibody

PIWIL1/HIWI antibody

Boster Bio Anti-PIWI-L1 Antibody (Catalog # A03159). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A03159
Size: 0.1 mg
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IF, IHC-P, WB

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Product Name

Anti-PIWI-L1 Antibody

View all PIWIL1/HIWI Antibodies

SKU/Catalog Number

A03159

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-PIWI-L1 Antibody (Catalog # A03159). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

PIWI-L1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-PIWI-L1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03159)

Host

Rabbit

Contents

PIWI-L1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

SK7

Isotype

IgG

Immunogen

PIWI-L1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PIWI-L1. The immunogen is located within amino acids 60 - 110 of PIWI-L1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least three isoforms of SYNPO are known to exist; this antibody will detect all three isoforms. SYNPO antibody is predicted to not cross-react with other SYNPO family member proteins.

Reactive Species

A03159 is reactive to PIWIL1 in Human, Mouse, Rat

Applications

A03159 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

98.603kDa

Background of PIWIL1/HIWI

PIWI-L1 is a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that are crucial for the biogenesis and function of small non-coding RNAs and play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. PIWI-L1 is thought to play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells as removing PIWI-L1 protein from single germline stem cells significantly decreases the rate of their division. Recent studies have shown that overexpression of PIWI-L1 (as well as other PIWI-like proteins) are potential biomarkers for colon and other cancers.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

PIWI-L1 antibody can be used for detection of PIWI-L1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For PIWIL1 (Source: Uniprot.org, NCBI)

Gene Name

PIWIL1

Full Name

Piwi-like protein 1

Weight

98.603kDa

Superfamily

argonaute family

Alternative Names

HIWI; HIWIpiwi homolog; MIWI; piwi (Drosophila)-like 1; PIWI; PIWIL1; piwi-like 1 (Drosophila); piwi-like protein 1 PIWIL1 CT80.1, HIWI, MIWI, PIWI piwi like RNA-mediated gene silencing 1 piwi-like protein 1|piwi homolog

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PIWIL1, check out the PIWIL1 Infographic

PIWIL1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PIWIL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A03159-0.1 mg

0.1 mg

$449

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