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Product Info Summary
| SKU: | A16310 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-PLEKHM3 Antibody
SKU/Catalog Number
A16310
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PLEKHM3 Antibody (Catalog # A16310). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
PLEKHM3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-PLEKHM3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16310)
Host
Rabbit
Contents
PLEKHM3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
PLEKHM3 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human PLEKHM3. The immunogen is located within the last 50 amino acids of PLEKHM3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least four isoforms of IFLTD1 are known to exist; this antibody will detect all but isoform 4
Reactive Species
A16310 is reactive to Plekhm3 in Human, Mouse, Rat
Applications
A16310 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
86.553kDa
Background of Plekhm3
PLEKHM3, also known as DAPR, is a member of the M family of Pleckstrin homology domain-containing proteins. PLEKHM3 was initially identified through chromatin immunoprecipitation and CpG microarray analysis examining proteins regulated by myocyte-enhancing factor 2. In C2C12 myoblast cells, PLEKHM3 binds to the PI3K signaling member protein kinase B in the cytosol prior to differentiation into myotubes. Following the initiation of differentiation, PLEKHM3 was also found in membrane fractions. Knockdown of PLEKHM3 expression by RNAi resulted in the inhibition of myotube formation, suggesting that PLEKHM3 is a key component required by myoblasts for orchestrating their differentiation during myogenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PLEKHM3 antibody can be used for detection of PLEKHM3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PLEKHM3 in mouse skeletal muscle tissue lysate with PLEKHM3 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of PLEKHM3 in human brain tissue with PLEKHM3 antibody at 5 μg/mL.
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Immunofluorescence of PLKHM3 in human brain tissue with PLKHM3 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For Plekhm3 (Source: Uniprot.org, NCBI)
Gene Name
Plekhm3
Full Name
Pleckstrin homology domain-containing family M member 3
Weight
86.553kDa
Alternative Names
DAPR; pleckstrin homology domain containing, family M, member 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Plekhm3, check out the Plekhm3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Plekhm3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PLEKHM3 Antibody (A16310)
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No publications found for A16310
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Customer Reviews
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