Anti-PLXNC1 Antibody Picoband™

Product Name

Anti-PLXNC1 Antibody Picoband™

View all Plexin C1 Antibodies

SKU/Catalog Number

A10941-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PLXNC1 Antibody Picoband™ catalog # A10941-1. Tested in WB, FCM, ELISA applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PLXNC1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10941-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human PLXNC1 recombinant protein (Position: K420-R819). Human PLXNC1 shares 78.6% amino acid (aa) sequence identity with mouse PLXNC1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A10941-1 is reactive to PLXNC1 in Human

Applications

A10941-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

220 kDa

Calculated molecular weight

175.742kDa

Background of Plexin C1

This gene encodes a member of the plexin family. Plexins are transmembrane receptors for semaphorins, a large family of proteins that regulate axon guidance, cell motility and migration, and the immune response. The encoded protein and its ligand regulate melanocyte adhesion, and viral semaphorins may modulate the immune response by binding to this receptor. The encoded protein may be a tumor suppressor protein for melanoma. Alternatively spliced transcript variants have been observed for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg /1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PLXNC1 using anti-PLXNC1 antibody (A10941-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human THP-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLXNC1 antigen affinity purified polyclonal antibody (Catalog # A10941-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLXNC1 at approximately 220 kDa. The expected band size for PLXNC1 is at 176 kDa.

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Figure 2. Flow Cytometry analysis of HepG2 cells using anti-PLXNC1 antibody (A10941-1).
Overlay histogram showing HepG2 cells stained with A10941-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PLXNC1 Antibody (A10941-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For PLXNC1 (Source: Uniprot.org, NCBI)

Alternative Names

CD232 antigen; CD232; plexin (semaphorin receptor); Plexin C1; PLXNC1; PLXN-C1; receptor for viral semaphorin protein; receptor for virally-encoded semaphorin; VESPR; VESPRplexin-C1; Virus-encoded semaphorin protein receptor PLXNC1 CD232, PLXN-C1, VESPR plexin C1 plexin-C1|plexin (semaphorin receptor)|receptor for viral semaphorin protein|receptor for virally-encoded semaphorin|virus-encoded semaphorin protein receptor

For more info on PLXNC1, check out the PLXNC1 Infographic

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In this infographic, you will see the following information for PLXNC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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