Anti-POLE1 Antibody

DNA Polymerase epsilon catalytic subunit A antibody

Boster Bio Anti-POLE1 Antibody (Catalog# A30644). Tested in IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: A30644
Size: 100ul
Reactive Species: Human, Rat
Host: Rabbit
Application: ELISA, IHC-P, IF (paraffin)

Product Name

Anti-POLE1 Antibody

View all DNA Polymerase epsilon catalytic subunit A Antibodies

SKU/Catalog Number

A30644

Size

100ul

Form

Liquid

Description

Boster Bio Anti-POLE1 Antibody (Catalog# A30644). Tested in IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-POLE1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30644)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A peptide derived from human POLE1. Immunogen sequence location: 1261-1310

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A30644 is reactive to POLE in Human, Rat

Applications

A30644 is guaranteed for ELISA, IHC-P, IF (paraffin) Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

261.518kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry, 1:100 - 1:300
ELISA, 1:40000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For POLE (Source: Uniprot.org, NCBI)

Gene Name

POLE

Full Name

DNA polymerase epsilon catalytic subunit A

Weight

261.518kDa

Superfamily

DNA polymerase type-B family

Alternative Names

DKFZp434F222; DNA polymerase epsilon catalytic subunit A; DNA polymerase II subunit A; EC 2.7.7; EC 2.7.7.7; POLE1FLJ21434; polymerase (DNA directed), epsilon POLE CRCS12, FILS, IMAGEI1, POLE DNA polymerase epsilon, catalytic subunit DNA polymerase epsilon catalytic subunit A|3-5 exodeoxyribonuclease|DNA polymerase II subunit A|DNA polymerase epsilon catalytic subunit protein|polymerase (DNA directed), epsilon, catalytic subunit|polymerase (DNA) epsilon, catalytic subunit

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on POLE, check out the POLE Infographic

POLE infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for POLE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A30644 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wang Ph, Gu Zh, Wan Dh, Zhu Wb, Qiu W, Weng Sp, Yu Xq, He Jg. Plos One. 2013;8(2):E52088. Doi: 10.1371/Journal.Pone.0052088. Epub 2013 Feb 6. Litopenaeus Vannamei Sterile-Alpha And Armadillo Motif Containing Protein (Lvsarm) Is Involved In Regulat...

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3 Customer Q&As for Anti-POLE1 Antibody

Question

I see that the anti-POLE1 antibody A30644 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-05-03

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-05-03

Question

We are currently using anti-POLE1 antibody A30644 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2017-08-15

Answer

The anti-POLE1 antibody (A30644) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-08-15

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic kidney using anti-POLE1 antibody A30644. Let me know if you need anything else.

S. Roberts

Verified customer

Asked: 2013-11-05

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-11-05

Order DetailsPrice
A30644

100 μL

$335

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