Anti-POLK/Dna Polymerase Kappa Antibody

Product Name

Anti-POLK/Dna Polymerase Kappa Antibody

View all DNA Polymerase Kappa Antibodies

SKU/Catalog Number

A01987

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-POLK/Dna Polymerase Kappa Antibody (Catalog # A01987). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-POLK/Dna Polymerase Kappa Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01987)

Host

Goat

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity-purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 817-830 of Human POLK (polymerase (DNA directed) kappa).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A01987 is reactive to Polk in Human

Applications

A01987 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

96.003kDa

Background of DNA Polymerase Kappa

POLK (also known as DNA polymerase kappa, DINB protein and DINP) is involved in DNA repair. POLK plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. POLK lacks 3'-5' proofreading exonuclease activity. POLK is a nuclear protein that binds to REV1L and PCNA. POLK is detected throughout the nucleus and at replication foci. Multiple alternative splice variants have been detected for this protein. POLK is found at low levels in testis, spleen, prostate and ovary and at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2,000 - 1:10,000
WB: 1:500 - 1:3,000

Validation Images & Assay Conditions

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Western blot analysis of POLK expression in HELA whole cell lysates (lane 1). POLK at 98KD was detected using goat anti-POLK Antigen Affinity purified polyclonal antibody (Catalog # A01987) at 1:1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1003).

Gene/Protein Information For Polk (Source: Uniprot.org, NCBI)

Alternative Names

DINB protein; DINPPOLQDINB1EC 2.7.7.7; DNA polymerase kappa; polymerase (DNA directed) kappa Polk|DINP, Din, Dinb1|polymerase (DNA directed), kappa|DNA polymerase kappa|DINB protein|DNA damage-inducible protein b|DNA-directed DNA polymerase kappa|DinB homolog 1

For more info on Polk, check out the Polk Infographic

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In this infographic, you will see the following information for Polk: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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