Anti-ELG-1 ATAD5 Antibody

Product Name

Anti-ELG-1 ATAD5 Antibody

View all ATAD5 Antibodies

SKU/Catalog Number

A06674

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-ELG-1 ATAD5 Antibody (Catalog # A06674). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-ELG-1 ATAD5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06674)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 63-76 of Human Elg 1.

Reactive Species

A06674 is reactive to ATAD5 in Human

Observed Molecular Weight

42 kDa

Calculated molecular weight

207.6 kDa

Background of ATAD5

ELG1 (also known as ATP(GTP)-binding protein or Chromosome fragility associated gene 1) is involved in a novel RFC complex that is probably involved in DNA damage and repair by ensuring replication fidelity. This antibody detects a band at about 120kDa in Hela, A431, Jurkat and HEK193 cells. This corresponds to the band size seen in Kanellis P et al. It remains unclear why the band size detected is much less than the 207kDa predicted in the protein sequence corresponding to CACC44537.2 (Q96QE3), but as our results correspond to those seen in Kanellis P et al. it is likely that the 120 kDa target is ELG1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06674 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:3,000 - 1:15,000
WB: 1:500 - 1:2,000
This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 120 kDa in size corresponding to Elg1 by western blotting in the appropriate cell lysate or extract.

Validation Images & Assay Conditions

See full target information ATAD5

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