Anti-POLR1D Antibody Picoband™

Product Name

Anti-POLR1D Antibody Picoband™

View all Polr1d Antibodies

SKU/Catalog Number

A07808-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-POLR1D Antibody Picoband™ catalog # A07808-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-POLR1D Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07808-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human POLR1D recombinant protein (Position: K10-F133). Human POLR1D shares 90.3% amino acid (aa) sequence identity with mouse POLR1D.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07808-1 is reactive to Polr1d in Human, Mouse, Rat

Applications

A07808-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

20 kDa

Calculated molecular weight

15.072kDa

Background of Polr1d

DNA-directed RNA polymerases I and III subunit RPAC2 is a protein that in humans is encoded by the POLR1D gene. The protein encoded by this gene is a component of the RNA polymerase I and RNA polymerase III complexes, which function in the synthesis of ribosomal RNA precursors and small RNAs, respectively. Mutations in this gene are a cause of Treacher Collins syndrome (TCS), a craniofacial development disorder. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of POLR1D using anti-POLR1D antibody (A07808-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human HEL whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR1D antigen affinity purified polyclonal antibody (Catalog # A07808-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLR1D at approximately 20 kDa. The expected band size for POLR1D is at 15 kDa.

Gene/Protein Information For Polr1d (Source: Uniprot.org, NCBI)

Alternative Names

AC19; DNA-directed RNA polymerase I subunit D; DNA-directed RNA polymerases I and III subunit RPAC2; FLJ20616; hRPA19; MGC9850; POLR1C; polymerase (RNA) I polypeptide D, 16kDa; RPA16RNA polymerase I 16 kDa subunit; RPA9; RPAC2RNA polymerases I and III subunit AC2; RPC16; RPO1-3; TCS2

For more info on Polr1d, check out the Polr1d Infographic

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In this infographic, you will see the following information for Polr1d: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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