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Product Info Summary
| SKU: | A09040 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-POU2F3 Antibody
SKU/Catalog Number
A09040
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-POU2F3 Antibody (Catalog # A09040). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
POU2F3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-POU2F3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09040)
Host
Rabbit
Contents
POU2F3 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
POU2F3 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human POU2F3. The immunogen is located within amino acids 370 - 420 of POU2F3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
POU2F3 antibody is human, mouse and rat reactive. At least two isoforms are known to exist. This antibody will recognize both isoforms. POU2F3 antibody is predicted to not cross-react with other members of the POU domain class 2 family.
Reactive Species
A09040 is reactive to Pou2f3 in Human, Mouse, Rat
Applications
A09040 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
47.071kDa
Background of Pou2f3
POU2F3, also known as Epoc-1, is a member of a family of POU domain family of transcription factors (1). POU2F3 is expressed primarily in the epidermis and plays a critical role in keratinocyte proliferation and differentiation (1,2). It is a crucial transcription factor that is required for the development of sweet, umami, and bitter, but not sour taste receptor cells (3). POU2F3 is also a candidate tumor suppressor protein, and aberrant promoter methylation of this gene may play a role in cervical cancer (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
POU2F3 antibody can be used for detection of POU2F3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of POU2F3 in SK-N-SH cell lysate with POU2F3 antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of POU2F3 in rat brain tissue with POU2F3 antibody at 5 μg/mL.
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Immunofluorescence of POU2F3 in rat brain tissue with POU2F3 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For Pou2f3 (Source: Uniprot.org, NCBI)
Gene Name
Pou2f3
Full Name
POU domain, class 2, transcription factor 3
Weight
47.071kDa
Superfamily
POU transcription factor family
Alternative Names
Epoc-1; MGC126698; oct-11; OCT11FLJ40063; Octamer-binding protein 11; Octamer-binding transcription factor 11; OTF11; OTF-11; PLA1; PLA-1; POU class 2 homeobox 3; POU domain class 2, transcription factor 3; POU domain, class 2, transcription factor 3; POU transcription factor; Skn-1a; Transcription factor PLA-1; Transcription factor Skn-1 Pou2f3|Epoc, Epoc-1, Oct-1, Oct-11a, Oct1, Oct11, Otf-1, Otf-11, Otf1, Otf11, S, Sk, Ski, Skin, Skin-1a, Skn, Skn-1a, Skn-li|POU domain, class 2, transcription factor 3|POU domain, class 2, transcription factor 3|oct-11|octamer-binding protein 11|octamer-binding transcription factor 11
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Pou2f3, check out the Pou2f3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Pou2f3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-POU2F3 Antibody (A09040)
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