Anti-PPP1R16B/Timap Antibody

PPP1R16B antibody

Boster Bio Anti-PPP1R16B/Timap Antibody (Catalog # A09221). Tested in WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A09221
Size: 100 µl
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

Product Name

Anti-PPP1R16B/Timap Antibody

View all PPP1R16B Antibodies

SKU/Catalog Number

A09221

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-PPP1R16B/Timap Antibody (Catalog # A09221). Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PPP1R16B/Timap Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09221)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human PPP1R16B.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A09221 is reactive to PPP1R16B in Human, Mouse

Applications

A09221 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

63.551kDa

Background of PPP1R16B

Regulator of protein phosphatase 1 (PP1) that acts as a positive regulator of pulmonary endothelial cell (EC) barrier function. Involved in PKA-mediated moesin dephosphorylation which is important in EC barrier protection against thrombin stimulation. Promotes the interaction of PPP1CA with RPSA/LAMR1 and in turn facilitates the dephosphorylation of RPSA/LAMR1. Involved in the regulation of endothelial cell filopodia extension. May be a downstream target for TGF-beta1 signaling cascade in endothelial cells.

Cao W., Am. J. Physiol. 283:C327-C337(2002).
Nagase T., DNA Res. 5:355-364(1998).
Nakajima D., DNA Res. 9:99-106(2002).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For PPP1R16B (Source: Uniprot.org, NCBI)

Gene Name

PPP1R16B

Full Name

Protein phosphatase 1 regulatory inhibitor subunit 16B

Weight

63.551kDa

Alternative Names

ANKRD4TIMAPankyrin repeat domain protein 4; Ankyrin repeat domain-containing protein 4; CAAX box protein TIMAP; hTIMAP; KIAA0823TGF-beta-inhibited membrane-associated protein; protein phosphatase 1 regulatory inhibitor subunit 16B; protein phosphatase 1, regulatory (inhibitor) subunit 16B PPP1R16B ANKRD4, TIMAP protein phosphatase 1 regulatory subunit 16B protein phosphatase 1 regulatory inhibitor subunit 16B|CAAX box protein TIMAP|TGF-beta-inhibited membrane-associated protein|ankyrin repeat domain protein 4|ankyrin repeat domain-containing protein 4|hTIMAP|protein phosphatase 1, regulatory (inhibitor) subunit 16B

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PPP1R16B, check out the PPP1R16B Infographic

PPP1R16B infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PPP1R16B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-PPP1R16B/Timap Antibody

Question

I was wanting to use your anti-PPP1R16B/Timap antibody for WB for mouse brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain identification?

Verified Customer

Verified customer

Asked: 2019-10-09

Answer

As indicated on the product datasheet, A09221 anti-PPP1R16B/Timap antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-09

Question

My lab would like to test anti-PPP1R16B/Timap antibody A09221 on mouse brain for research purposes, then I may be interested in using anti-PPP1R16B/Timap antibody A09221 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-06-18

Answer

The products we sell, including anti-PPP1R16B/Timap antibody A09221, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-06-18

Question

We are currently using anti-PPP1R16B/Timap antibody A09221 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2018-12-21

Answer

The anti-PPP1R16B/Timap antibody (A09221) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-12-21

Order DetailsPrice
A09221-100uL

100uL

$360

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