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Product Info Summary
| SKU: | A15169 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Protein shisa-9 SHISA9 Antibody
SKU/Catalog Number
A15169
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein shisa-9 SHISA9 Antibody (Catalog # A15169). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SHISA9 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Protein shisa-9 SHISA9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15169)
Host
Rabbit
Contents
SHISA9 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SHISA9 antibody was raised against a 22 amino acid synthetic peptide near the center of human SHISA9. The immunogen is located within amino acids 120 - 170 of SHISA9.
Reactive Species
A15169 is reactive to SHISA9 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
46.9 kDa
Background of SHISA9
Shisa proteins are a recently-identified family of modulators of both FGF and Wnt signaling that block both maturation and transport to the cell surface of their respective receptors. SHISA9, also known as CKAMP44a, is a brain-specific type I transmembrane protein that associates with AMPA receptors in synaptic spines and promotes AMPA receptor desensitization at excitatory synapses. It is thought to modulate short-term plasticity at specific excitatory synapses.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15169 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SHISA9 antibody can be used for detection of SHISA9 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SHISA9 in rat brain tissue lysate with SHISA9 antibody at 1 μg/mL.
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Immunohistochemistry of SHISA9 in human brain tissue with SHISA9 antibody at 2.5 μg/mL.
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Immunofluorescence of SHISA9 in human brain tissue with SHISA9 antibody at 20 μg/mL.
Specific Publications For Anti-Protein shisa-9 SHISA9 Antibody (A15169)
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