Anti-Protein Wnt-10b Wnt10b Antibody

Product Name

Anti-Protein Wnt-10b Wnt10b Antibody

View all WNT10B Antibodies

SKU/Catalog Number

A02574

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Protein Wnt-10b Wnt10b Antibody (Catalog # A02574). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Wnt10b antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Protein Wnt-10b Wnt10b Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02574)

Host

Rabbit

Contents

Wnt10b Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Wnt10b antibody was raised against a 15 amino acid synthetic peptide from near the center of human Wnt10b. The immunogen is located within amino acids 130 - 180 of Wnt10b.

Cross-reactivity

Two isoforms of Wnt10b are known to exist; this antibody will only recognize the longer isoform. This Wnt10b antibody will not cross-react with Wnt10a.

Reactive Species

A02574 is reactive to WNT10B in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

43.0 kDa

Background of WNT10B

Wnt10b is a member of the Wnt family, a gene family that encodes secreted signaling proteins that play crucial roles in normal development such as regulation of cell fate and patterning during embryogenesis as well as neoplastic transformation. Wnt10b is found in the mouse mammary tumor virus insertion site where it is activated and causes mammary tumors. Elevated levels of Wnt10b have also been detected in human breast carcinomas. Wnt10b is known to be involved in adipogenesis, maintaining the preadipocyte in an undifferentiated state. More recently, Wnt10b has been shown to promote epithelial cell differentiation and hair shaft growth, demonstrating that Wnt10b has multiple roles in cell growth and differentiation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02574 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Wnt10b antibody can be used for detection of Wnt10b by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information WNT10B

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