Product Info Summary
SKU: | M01083 |
---|---|
Size: | 100ug/vial |
Reactive Species: | Human, Horse |
Host: | Mouse |
Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Proximal Nephrogenic Antigen (Renal Cell Marker) Monoclonal Antibody
View all Carbonic Anhydrase IX/CA9 Antibodies
SKU/Catalog Number
M01083
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Proximal Nephrogenic Antigen (Renal Cell Marker) Monoclonal Antibody (Catalog # M01083). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Horse.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Proximal Nephrogenic Antigen (Renal Cell Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01083)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: PN-15
Isotype
IgG2b, kappa
Immunogen
Microsomal fraction of human renal cortical tissue homogenate
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M01083 is reactive to CA9 in Human, Horse
Applications
M01083 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
49.698kDa
Background of Carbonic Anhydrase IX/CA9
Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This monoclonal antibody may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Reconstitute with distilled water.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Anti-PNA Mouse Monoclonal Antibody (PN-15).
Protein Target Info & Infographic
Gene/Protein Information For CA9 (Source: Uniprot.org, NCBI)
Gene Name
CA9
Full Name
Carbonic anhydrase 9
Weight
49.698kDa
Superfamily
alpha-carbonic anhydrase family
Alternative Names
CA9; CAIX; CA-IX; Carbonate dehydratase IX; carbonic anhydrase 9; Carbonic Anhydrase IX; carbonic dehydratase; EC 4.2.1.1; G250; Membrane antigen MN; MN; P54/58N; PMW1; RCC; RCC-associated antigen G250; RCC-associated protein G250; Renal cell carcinoma-associated antigen G250 CA9 CAIX, MN carbonic anhydrase 9 carbonic anhydrase 9|CA-IX|P54/58N|RCC-associated antigen G250|RCC-associated protein G250|carbonate dehydratase IX|carbonic anhydrase IX|carbonic dehydratase|membrane antigen MN|pMW1|renal cell carcinoma-associated antigen G250
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CA9, check out the CA9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CA9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Proximal Nephrogenic Antigen (Renal Cell Marker) Monoclonal Antibody (M01083)
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No publications found for M01083
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Proximal Nephrogenic Antigen (Renal Cell Marker) Monoclonal Antibody
Question
I am interested in to test anti-Proximal Nephrogenic antigen (Renal Cell Marker) Monoclonal antibody M01083 on human carcinoma for research purposes, then I may be interested in using anti-Proximal Nephrogenic antigen (Renal Cell Marker) Monoclonal antibody M01083 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-11-01
Answer
The products we sell, including anti-Proximal Nephrogenic antigen (Renal Cell Marker) Monoclonal antibody M01083, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-11-01
Question
Is a blocking peptide available for product anti-Proximal Nephrogenic antigen (Renal Cell Marker) Monoclonal antibody (M01083)?
Verified Customer
Verified customer
Asked: 2018-03-01
Answer
We do provide the blocking peptide for product anti-Proximal Nephrogenic antigen (Renal Cell Marker) Monoclonal antibody (M01083). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-03-01
Question
Would anti-Proximal Nephrogenic antigen (Renal Cell Marker) Monoclonal antibody M01083 work for IHC with carcinoma?
A. Jones
Verified customer
Asked: 2013-04-04
Answer
According to the expression profile of carcinoma, CA9 is highly expressed in carcinoma. So, it is likely that anti-Proximal Nephrogenic antigen (Renal Cell Marker) Monoclonal antibody M01083 will work for IHC with carcinoma.
Boster Scientific Support
Answered: 2013-04-04