Anti-PRP19 PRPF19 Rabbit Monoclonal Antibody

PRP19 antibody

Boster Bio Anti-PRP19 PRPF19 Rabbit Monoclonal Antibody catalog # M02434. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M02434
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-PRP19 PRPF19 Rabbit Monoclonal Antibody

View all PRP19 Antibodies

SKU/Catalog Number

M02434

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-PRP19 PRPF19 Rabbit Monoclonal Antibody catalog # M02434. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PRP19 PRPF19 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02434)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

FEC-16

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human PRP19

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M02434 is reactive to PRPF19 in Human, Mouse, Rat

Applications

M02434 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

24 kDa

Calculated molecular weight

55.181kDa

Background of PRP19

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For PRPF19 (Source: Uniprot.org, NCBI)

Gene Name

PRPF19

Full Name

Pre-mRNA-processing factor 19

Weight

55.181kDa

Superfamily

WD repeat PRP19 family

Alternative Names

hPSO4; NMP200SNEV; Nuclear matrix protein 200; nuclear matrix protein NMP200 related to splicing factor PRP19; pre-mRNA-processing factor 19; PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae); PRP19PRP19/PSO4 homolog (S. cerevisiae); PSO4PRP19/PSO4 homolog; Senescence evasion factor; UBOX4 PRPF19 NMP200, PRP19, PSO4, SNEV, UBOX4, hPSO4 pre-mRNA processing factor 19 pre-mRNA-processing factor 19|PRP19/PSO4 homolog|PRP19/PSO4 pre-mRNA processing factor 19 homolog|RING-type E3 ubiquitin transferase PRP19|nuclear matrix protein 200|nuclear matrix protein NMP200 related to splicing factor PRP19|psoralen 4|senescence evasion factor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PRPF19, check out the PRPF19 Infographic

PRPF19 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PRPF19: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

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3 Customer Q&As for Anti-PRP19 PRPF19 Rabbit Monoclonal Antibody

Question

Will anti-PRP19 Rabbit Monoclonal antibody M02434 work on bovine IF with frontal cortex?

Verified Customer

Verified customer

Asked: 2019-08-06

Answer

Our lab technicians have not tested anti-PRP19 Rabbit Monoclonal antibody M02434 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-PRP19 Rabbit Monoclonal antibody M02434 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine frontal cortex in IF, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-06

Question

I see that the anti-PRP19 Rabbit Monoclonal antibody M02434 works with IF, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-07-27

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-07-27

Question

We are currently using anti-PRP19 Rabbit Monoclonal antibody M02434 for mouse tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2017-12-04

Answer

The anti-PRP19 Rabbit Monoclonal antibody (M02434) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-12-04

Order DetailsPrice
M02434

100μL

$370
M02434-30ul

30μL sample

$99
M02434-carrier-free

Carrier Free

$370

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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