Product Info Summary
SKU: | A03018 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Monkey, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Rac GAP1 Antibody
View all MgcRacGAP/RACGAP1 Antibodies
SKU/Catalog Number
A03018
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Rac GAP1 Antibody catalog # A03018. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rac GAP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03018)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Rac GAP1 around the non-phosphorylation site of S387.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03018 is reactive to Racgap1 in Human, Monkey, Mouse, Rat
Applications
A03018 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
70.158kDa
Background of MgcRacGAP/RACGAP1
Anti-Cul2 antibody can be used in western blotting, ELISA and IP. Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 2 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul2 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul2 may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Cul2 is part of the SCF complex consisting of CUL1, RBX1, SKP1 and SKP2, where it interacts directly with SKP1, SKP2 and RBX1. Cul2 also interacts with RNF7 and is part of a complex with TIP120A/CAND1 and RBX1. The unneddylated form interacts with TIP120A/CAND1 and the interaction negatively regulates the association with SKP1 in the SCF complex.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of specific cells using Rac GAP1 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For Racgap1 (Source: Uniprot.org, NCBI)
Gene Name
Racgap1
Full Name
Rac GTPase-activating protein 1
Weight
70.158kDa
Alternative Names
GTPase activating protein; HsCYK-4; ID-GAP; KIAA1478; Male germ cell RacGap; MgcRacGAP; MgcRacGAPrac GTPase-activating protein 1; Rac GTPase activating protein 1; RACGAP1 Racgap1|AI227039, AI327394, Band, Band25, GTP, GTPase, MgcRa, MgcRacGAP, gtl1, gtl11, mKIAA1478|Rac GTPase-activating protein 1|rac GTPase-activating protein 1|male germ cell RacGap
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Racgap1, check out the Racgap1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Racgap1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rac GAP1 Antibody (A03018)
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No publications found for A03018
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Anti-Rac GAP1 Antibody
Question
Please see the WB image, lot number and protocol we used for placenta {eco:0000312 using anti-Rac GAP1 antibody A03018. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-20
Question
I see that the anti-Rac GAP1 antibody A03018 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-08
Question
Does A03018 anti-Rac GAP1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
It shows on the product datasheet, A03018 anti-Rac GAP1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-15
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta {eco:0000312 using anti-Rac GAP1 antibody A03018. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-20
Question
We are currently using anti-Rac GAP1 antibody A03018 for monkey tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, monkey, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-21
Answer
The anti-Rac GAP1 antibody (A03018) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-21
Question
Is a blocking peptide available for product anti-Rac GAP1 antibody (A03018)?
Verified Customer
Verified customer
Asked: 2018-02-16
Answer
We do provide the blocking peptide for product anti-Rac GAP1 antibody (A03018). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-02-16
Question
We are interested in to test anti-Rac GAP1 antibody A03018 on monkey placenta {eco:0000312 for research purposes, then I may be interested in using anti-Rac GAP1 antibody A03018 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Wu
Verified customer
Asked: 2013-12-05
Answer
The products we sell, including anti-Rac GAP1 antibody A03018, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-12-05