Click image to see more details
Product Info Summary
| SKU: | A03328-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-RAD54 RAD54L Antibody
SKU/Catalog Number
A03328-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-RAD54 RAD54L Antibody (Catalog# A03328-1). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RAD54 RAD54L Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03328-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human RAD54L. Immunogen sequence location: 221-270
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03328-1 is reactive to RAD54L in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
84.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03328-1 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:5000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
Click image to see more details
Western blot analysis of lysates from HeLa cells, Jurkat cells, and 293 cells, using RAD54 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-RAD54 RAD54L Antibody (A03328-1)
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Customer Q&As
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6 Customer Q&As for Anti-RAD54 RAD54L Antibody
Question
We are currently using anti-RAD54 antibody A03328-1 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
The anti-RAD54 antibody (A03328-1) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-30
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-RAD54 antibody A03328-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-10
Question
We need to test anti-RAD54 antibody A03328-1 on mouse erythroleukemia for research purposes, then I may be interested in using anti-RAD54 antibody A03328-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
G. Zhao
Verified customer
Asked: 2019-08-09
Answer
The products we sell, including anti-RAD54 antibody A03328-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-09
Question
Would anti-RAD54 antibody A03328-1 work for WB with erythroleukemia?
Verified Customer
Verified customer
Asked: 2018-08-15
Answer
According to the expression profile of erythroleukemia, RAD54L is highly expressed in erythroleukemia. So, it is likely that anti-RAD54 antibody A03328-1 will work for WB with erythroleukemia.
Boster Scientific Support
Answered: 2018-08-15
Question
See below the WB image, lot number and protocol we used for erythroleukemia using anti-RAD54 antibody A03328-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-10-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-26
Question
I have a question about product A03328-1, anti-RAD54 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-09-11
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03328-1 anti-RAD54 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-09-11
