Anti-RAD54 RAD54L Antibody

Product Name

Anti-RAD54 RAD54L Antibody

View all RAD54L Antibodies

SKU/Catalog Number

A03328-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-RAD54 RAD54L Antibody (Catalog# A03328-1). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RAD54 RAD54L Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03328-1)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

7A7

Isotype

Rabbit IgG

Immunogen

A peptide derived from human RAD54L. Immunogen sequence location: 221-270

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A03328-1 is reactive to RAD54L in Human, Mouse

Applications

A03328-1 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

84.352kDa

Background of RAD54L

Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:5000
Not yet tested in other applications.

Validation Images & Assay Conditions

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Western blot analysis of lysates from HeLa cells, Jurkat cells, and 293 cells, using RAD54 Antibody. The lane on the right is blocked with the synthesized peptide.

Gene/Protein Information For RAD54L (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.6.1; hHR54EC 3.6.4.-; HR54; hRAD54HRAD54; RAD54 (S.cerevisiae)-like; RAD54 homolog; RAD54ADNA repair and recombination protein RAD54-like; RAD54-like (S. cerevisiae) RAD54L HR54, RAD54A, hHR54, hRAD54 RAD54 like DNA repair and recombination protein RAD54-like|RAD54 homolog

For more info on RAD54L, check out the RAD54L Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RAD54L: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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